Any feedback?
Literature summary extracted from
- Cloning and molecular characterization of the first aquatic hyaluronidase, SFHYA1, from the venom of stonefish (Synanceja horrida) (2005), Gene, 346, 71-81.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.35 | precursor from venom gland, DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence comparisons, functional expression of His-tagged enzyme in insect cells, but not in Escherichia coli, and secretion of the recombinant enzyme, overview | Synanceia horrida |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.35 | extracellular | venom | Synanceia horrida | - |
- |
| 3.2.1.35 | intracellular | venom gland enzyme | Synanceia horrida | 5622 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.35 | Synanceia horrida | Q801Z8 | stonefish | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.35 | glycoprotein | the enzyme contains three potential N-glycosylation sites | Synanceia horrida |
| 3.2.1.35 | proteolytic modification | the precursor enzyme contains a signal sequence for targeting into endoplasmic reticulum | Synanceia horrida |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.35 | native enzyme from lyophilized venom by affinity chromatography | Synanceia horrida |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.2.1.35 | hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide | the enzyme contains four hyaluronan binding sites [B(X)7B] | Synanceia horrida |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.35 | venom | - |
Synanceia horrida | - |
| 3.2.1.35 | venom gland | precursor | Synanceia horrida | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.35 | hyaluronan + H2O | human substrate | Synanceia horrida | hyaluronan oligomers | - |
? |  |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.35 | hyaluronidase | - |
Synanceia horrida |
| 3.2.1.35 | SFHYA1 | - |
Synanceia horrida |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
