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Literature summary extracted from

  • Fialho, M.B.; Carmona, E.C.
    Purification and characterization of xylanases from Aspergillus giganteus (2004), Folia Microbiol. (Praha), 49, 13-18.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 2,4-dithiothreitol stimulation, both xylanase I and xylanase II Aspergillus giganteus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Cu2+ strong, both xylanase I and xylanase II Aspergillus giganteus
3.2.1.8 Hg2+ strong, both xylanase I and xylanase II Aspergillus giganteus
3.2.1.8 sodium dodecylsulfate strong, both xylanase I and xylanase II Aspergillus giganteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 21000
-
 x * 21000, xylanase I, x * 24000, xylanase II, SDS-PAGE Aspergillus giganteus
3.2.1.8 24000
-
 x * 21000, xylanase I, x * 24000, xylanase II, SDS-PAGE Aspergillus giganteus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Aspergillus giganteus
-
 isoforms xylanase I, xylanase II, growth in xylan
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.8 glycoprotein xylanase I, about 56% carbohydtate content, xylanase II, about 62% carbohydrate Aspergillus giganteus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 isoforms xylanase I, xylanase II Aspergillus giganteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 xylan + H2O oatspelt xylan is preferred substrate Aspergillus giganteus xylotriose + xylotetraose + larger xylooligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 21000, xylanase I, x * 24000, xylanase II, SDS-PAGE Aspergillus giganteus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 50
-
 both xylanase I and xylanase II Aspergillus giganteus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 50
-
 xylanase I, half-life 22.5 min, xylanase II, half-life 17.5 min Aspergillus giganteus
3.2.1.8 60
-
 xylanase I, half-life 1.5 min, xylanase II, half-life 1.5 min Aspergillus giganteus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 407
-
 xylan xylanase II, birchwood xylan, pH 6.0, 50°C Aspergillus giganteus
3.2.1.8 468
-
 xylan xylanase II, oatspelt xylan, pH 6.0, 50°C Aspergillus giganteus
3.2.1.8 1031
-
 xylan xylanase I, birchwood xylan, pH 6.0, 50°C Aspergillus giganteus
3.2.1.8 1345
-
 xylan xylanase I, oatspelt xylan, pH 6.0, 50°C Aspergillus giganteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6 6.5 xylanase I Aspergillus giganteus
3.2.1.8 6
-
 xylanase II Aspergillus giganteus