Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mammeri, H.; Poirel, L.; Nazik, H.; Nordmann, P.
    Cloning and functional characterization of the ambler class C beta-lactamase of Yersinia ruckeri (2006), FEMS Microbiol. Lett., 257, 57-62.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expression in Escherichia coli Yersinia ruckeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.001
-
 cefotaxime 30°C, pH 7.0 Yersinia ruckeri
3.5.2.6 0.001
-
 cefuroxime 30°C, pH 7.0 Yersinia ruckeri
3.5.2.6 0.001
-
 cefoxitin 30°C, pH 7.0 Yersinia ruckeri
3.5.2.6 0.05
-
 cephalothin 30°C, pH 7.0 Yersinia ruckeri
3.5.2.6 0.065
-
 benzylpenicillin 30°C, pH 7.0 Yersinia ruckeri
3.5.2.6 0.43
-
 cephaloridin 30°C, pH 7.0 Yersinia ruckeri
3.5.2.6 0.5
-
 ceftazidime 30°C, pH 7.0 Yersinia ruckeri

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Yersinia ruckeri Q20I87
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6
-
 Yersinia ruckeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 benzylpenicillin + H2O
-
 Yersinia ruckeri (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefotaxime + H2O
-
 Yersinia ruckeri (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefoxitin + H2O
-
 Yersinia ruckeri (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 ceftazidime + H2O
-
 Yersinia ruckeri (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cefuroxime + H2O
-
 Yersinia ruckeri (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Yersinia ruckeri (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephalothin + H2O
-
 Yersinia ruckeri (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 beta-lactamase YRC-1
-
 Yersinia ruckeri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.3
-
 cefotaxime 30°C, pH 7.0 Yersinia ruckeri
3.5.2.6 0.3
-
 cefoxitin 30°C, pH 7.0 Yersinia ruckeri
3.5.2.6 1
-
 ceftazidime 30°C, pH 7.0 Yersinia ruckeri
3.5.2.6 1
-
 cefuroxime 30°C, pH 7.0 Yersinia ruckeri
3.5.2.6 45
-
 benzylpenicillin 30°C, pH 7.0 Yersinia ruckeri
3.5.2.6 120
-
 cephaloridin 30°C, pH 7.0 Yersinia ruckeri
3.5.2.6 400
-
 cephalothin 30°C, pH 7.0 Yersinia ruckeri

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.2.6 Yersinia ruckeri isoelectric focusing
-
 8.3