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Literature summary extracted from

  • Oh, K.W.; Kim, M.J.; Kim, H.Y.; Kim, B.Y.; Baik, M.Y.; Auh, J.H.; Park, C.S.
    Enzymatic characterization of a maltogenic amylase from Lactobacillus gasseri ATCC 33323 expressed in Escherichia coli (2005), FEMS Microbiol. Lett., 252, 175-181.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.133 expression in Escherichia coli Lactobacillus gasseri

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.133 acarbose
-
 Lactobacillus gasseri
3.2.1.133 Co2+ 5 mM, 76% inhibition Lactobacillus gasseri
3.2.1.133 EDTA 5 mM, 74% inhibition Lactobacillus gasseri
3.2.1.133 Fe2+ 5 mM, 90% inhibition Lactobacillus gasseri
3.2.1.133 Zn2+ 5 mM, 96% inhibition Lactobacillus gasseri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.133 intracellular
-
 Lactobacillus gasseri 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.133 Ca2+ 5 mM, causes 20% increase in hydrolysis of starch Lactobacillus gasseri
3.2.1.133 Mg2+ 5 mM, causes 9% increase in hydrolysis of starch Lactobacillus gasseri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.133 65000
-
 4 * 65000, SDS-PAGE Lactobacillus gasseri
3.2.1.133 211000
-
 gel filtration Lactobacillus gasseri

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.133 Lactobacillus gasseri
-
 ATCC 33323
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.133 recombinant Lactobacillus gasseri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.133 58.7
-
 hydrolysis of beta-cyclodextrin Lactobacillus gasseri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.133 beta-cyclodextrin + H2O relative hydrolytic activity towards beta-cyclodextrin, soluble starch and pullulan are 8:1:1.9 Lactobacillus gasseri maltose + ?
-
 ?
3.2.1.133 additional information enzyme shows hydrolytic activity towards alpha-1,6-glycosidic linkage Lactobacillus gasseri ?
-
 ?
3.2.1.133 pullulan + H2O relative hydrolytic activity towards beta-cyclodextrin, soluble starch and pullulan are 8:1:1.9 Lactobacillus gasseri maltose + D-glucose + panose mainly maltose and glucose with relatively minor quantity of panose and other maltooligosaccharides ?
3.2.1.133 soluble starch + H2O relative hydrolytic activity towards beta-cyclodextrin, soluble starch and pullulan are 8:1:1.9 Lactobacillus gasseri maltose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.133 tetramer 4 * 65000, SDS-PAGE Lactobacillus gasseri

Synonyms

EC Number Synonyms Comment Organism
3.2.1.133 LGMA
-
 Lactobacillus gasseri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.133 55
-
 hydrolysis of beta-cyclodextrin, starch and pullulan Lactobacillus gasseri

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.133 30 60 30°C: about 20% of maximal activity with starch, about 25% of maximal activity with beta-cyclodextrin and about 35% of maximal activity with pullulan, 60°C: about 40% of maximal activity with pullulan, about 50% of maximal activity with starch anf about 80% of maximal activity with beta-cyclodextrin Lactobacillus gasseri