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Literature summary extracted from

  • Urich, T.; Kroke, A.; Bauer, C.; Seyfarth, K.; Reuff, M.; Kletzin, A.
    Identification of core active site residues of the sulfur oxygenase reductase from Acidianus ambivalens by site-directed mutagenesis (2005), FEMS Microbiol. Lett., 248, 171-176.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.13.11.55 degradation initial enzyme in the sulfur-oxidation pathway Acidianus ambivalens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.55 expression in Escherichia coli BL21 Acidianus ambivalens

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.55 C101/104A enzyme with decreased specific activity Acidianus ambivalens
1.13.11.55 C101/104S enzyme with decreased specific activity Acidianus ambivalens
1.13.11.55 C101A enzyme with decreased specific activity Acidianus ambivalens
1.13.11.55 C101A iron content is 49% of that of the recombinant wild-type enzyme, oxygenase activity is 12.8% of the activity of recombinant wild-type enzyme, reductase activity is 10.3% of the activity of recombinant wild-type enzyme Acidianus ambivalens
1.13.11.55 C101A/C104A iron content is 124% of that of the recombinant wild-type enzyme, oxygenase activity is 9.7% of the activity of recombinant wild-type enzyme, reductase activity is 15.3% of the activity of recombinant wild-type enzyme Acidianus ambivalens
1.13.11.55 C101S enzyme with decreased specific activity and a proportional decrease in iron content Acidianus ambivalens
1.13.11.55 C101S mutant enzyme contains no iron, oxygenase activity is 1.04% of the activity of recombinant wild-type enzyme, reductase activity is 0.5% of the activity of recombinant wild-type enzyme Acidianus ambivalens
1.13.11.55 C101S/C104S iron content is 89% of that of the recombinant wild-type enzyme, oxygenase activity is 19.8% of the activity of recombinant wild-type enzyme, reductase activity is 27.9% of the activity of recombinant wild-type enzyme Acidianus ambivalens
1.13.11.55 C104A enzyme with decreased specific activity Acidianus ambivalens
1.13.11.55 C104A iron content is 42% of that of the recombinant wild-type enzyme, oxygenase activity is 11.8% of the activity of recombinant wild-type enzyme, reductase activity is 5.5% of the activity of recombinant wild-type enzyme Acidianus ambivalens
1.13.11.55 C104S enzyme with decreased specific activity Acidianus ambivalens
1.13.11.55 C104S iron content is 67% of that of the recombinant wild-type enzyme, oxygenase activity is 19.8% of the activity of recombinant wild-type enzyme, reductase activity is 14.3% of the activity of recombinant wild-type enzyme Acidianus ambivalens
1.13.11.55 C31A inactive enzyme Acidianus ambivalens
1.13.11.55 C31A inactive mutant enzyme, iron content is similar to that of recombinant wild-type enzyme Acidianus ambivalens
1.13.11.55 C31S inactive enzyme Acidianus ambivalens
1.13.11.55 C31S inactive mutant enzyme, iron content is similar to that of recombinant wild-type enzyme Acidianus ambivalens
1.13.11.55 E114A inactive enzyme with no iron incorporated Acidianus ambivalens
1.13.11.55 E114A mutation results in inactive enzyme with no measurable iron found Acidianus ambivalens
1.13.11.55 E114D enzyme with 1% of wild type activity Acidianus ambivalens
1.13.11.55 E114D iron content is 4.4% of wild-type value, sulfur-oxidizing and sulfur-reducing activity is about 1% of the activity of activity of the recombinant wild-type enzyme Acidianus ambivalens
1.13.11.55 H86A inactive enzyme with no iron incorporated Acidianus ambivalens
1.13.11.55 H86A mutation results in inactive enzyme with no measurable iron found Acidianus ambivalens
1.13.11.55 H90A inactive enzyme with no iron incorporated Acidianus ambivalens
1.13.11.55 H90A mutation results in inactive enzyme with no measurable iron found Acidianus ambivalens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.55 Fe iron content: 0.45 mol per mol subunit for recombinant wild-type enzyme, below 0.1 mol per mol subunit for mutant enzyme H86A, below 0.02 mol per mol subunit for mutant enzyme H90A, below 0.01 mol per mol subunit for mutant enzyme E114A, 0.02 mol per mol subunit for mutant enzyme E114D, 0.47 mol per mol subunit for mutant enzyme C31A, 0.42 mol per mol subunit for mutant enzyme C31S, 0.22 mol per mol subunit for mutant enzyme C101A, below 0.03 mol per mol subunit for mutant enzyme C101S, 0.19 mol per mol subunit for mutant enzyme C104A, 0.3 mol per mol subunit for mutant enzyme C104S, 0.56 mol per mol subunit for mutant enzyme C101A/C104A, 0.4 mol per mol subunit for mutant enzyme C101S/C104S Acidianus ambivalens
1.13.11.55 Fe2+ mononucler non-heme iron site Acidianus ambivalens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.55 sulfur + H2O + O2 Acidianus ambivalens initial enzyme in the sulfur oxidation pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.55 Acidianus ambivalens
-
-
-
1.13.11.55 Acidianus ambivalens P29082
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.55 one-step procedure over Strep-Tactin columns Acidianus ambivalens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.55 0.02
-
 mutant C101S, U/mg, reductase reaction Acidianus ambivalens
1.13.11.55 0.03
-
 mutant E114D, U/mg, reductase reaction Acidianus ambivalens
1.13.11.55 0.11
-
 mutant E114D, U/mg, oxygenase reaction Acidianus ambivalens
1.13.11.55 0.12
-
 mutant C101S, U/mg, oxygenase reaction Acidianus ambivalens
1.13.11.55 0.23
-
 mutant C104A, U/mg, reductase reaction Acidianus ambivalens
1.13.11.55 0.43
-
 mutant C101A, U/mg, reductase reaction Acidianus ambivalens
1.13.11.55 0.6
-
 mutant C104S, U/mg, reductase reaction Acidianus ambivalens
1.13.11.55 0.64
-
 mutant C101/104A, U/mg, reductase reaction Acidianus ambivalens
1.13.11.55 1.12
-
 mutant C101/104A, U/mg, oxygenase reaction Acidianus ambivalens
1.13.11.55 1.17
-
 mutant C101/104S, U/mg, reductase reaction Acidianus ambivalens
1.13.11.55 1.35
-
 mutant C104A, U/mg, oxygenase reaction Acidianus ambivalens
1.13.11.55 1.47
-
 mutant C101, U/mg, oxygenase reaction Acidianus ambivalens
1.13.11.55 2.28
-
 mutant C104S, U/mg, oxygenase reaction Acidianus ambivalens
1.13.11.55 2.29
-
 mutant C101/104S, U/mg, oxygenase reaction Acidianus ambivalens
1.13.11.55 4.19
-
 wild type, U/mg, reductase reaction Acidianus ambivalens
1.13.11.55 11.52
-
 wild type, U/mg, oxygenase reaction Acidianus ambivalens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.55 sulfur + H2O + O2 initial enzyme in the sulfur oxidation pathway Acidianus ambivalens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.55 SOR
-
 Acidianus ambivalens
1.13.11.55 sulfur oxygenase reductase
-
 Acidianus ambivalens