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Literature summary extracted from

  • Ishikawa, Y.; Tokumitsu, H.; Inuzuka, H.; Murata-Hori, M.; Hosoya, H.; Kobayashi, R.
    Identification and characterization of novel components of a Ca2+/calmodulin-dependent protein kinase cascade in HeLa cells (2003), FEBS Lett., 550, 57-63.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.17 Calmodulin absolutely dependent on Homo sapiens
2.7.11.17 ionomycin activates the Ca2+/calmodulin-activated CaM-K1delta further 1.5fold Homo sapiens
2.7.11.17 additional information CaM-K1delta is activated about 30fold by phosphorylation through the Ca2+/calmodulin-dependent protein kinase kinase CaM-KKalpha Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.17 CaM-K1delta, DNA and amino acid sequence determination and analysis, expression of GST-tagged wild-type and mutant enzyme in Escherichia coli strain JM109, transient expression of HA-tagged CaM-K1delta 1-296 Ca2+/calmodulin-independent mutant in HeLa and COS-7 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.17 additional information CaM-K1delta 1-296 truncation mutant is catalytically active and Ca2+/calmodulin-independent, lacks the putative autoinhibitory domain and the Ca2+/calmodulin binding region, the truncation mutant is fully activated by CaM-KKalpha Homo sapiens
2.7.11.17 T180A site-directed mutagenesis, the mutant enzyme shows highly reduced activity compared to the wild-type enzyme, the mutant is phosphorylated and activated by CaM-KKalpha Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.17 STO-609 the CaM-KKalpha inhibitor STO-609 inhibits activity of the CaM-K1delta truncation mutant 1-296 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.17 Ca2+ absolutely dependent on Homo sapiens
2.7.11.17 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.17 additional information Homo sapiens components of the Ca2+/calmodulin-dependent protein kinase cascade, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.17 Homo sapiens Q8IU85 isozyme CaM-K1delta
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.17 phosphoprotein CaM-K1delta is activated about 30fold by phosphorylation through the Ca2+/calmodulin-dependent protein kinase kinase CaM-KKalpha, mechanism, CaM-K1delta performs autophosphorylation in a Ca2+/calmodulin-dependent manner which has no impact on its kinase activity Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.17 recombinant GST-tagged wild-type and mutant CaM-K1delta from Escherichia coli by glutathione affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.17 colon
-
 Homo sapiens
-
2.7.11.17 HeLa cell
-
 Homo sapiens
-
2.7.11.17 liver
-
 Homo sapiens
-
2.7.11.17 additional information CaM-K1delta tissue expression pattern, no expression in intestine, kidney, placenta, heart, and lung Homo sapiens
-
2.7.11.17 ovary
-
 Homo sapiens
-
2.7.11.17 pancreas
-
 Homo sapiens
-
2.7.11.17 prostate gland low expression level of CaM-K1delta Homo sapiens
-
2.7.11.17 skeletal muscle low expression level of CaM-K1delta Homo sapiens
-
2.7.11.17 spleen
-
 Homo sapiens
-
2.7.11.17 testis high expression level of CaM-K1delta Homo sapiens
-
2.7.11.17 thymus low expression level of CaM-K1delta Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.17 0.035
-
 purified recombinant CaM-K1delta Homo sapiens
2.7.11.17 0.98
-
 purified recombinant CaM-K1delta phosphorylated by CaM-KKalpha Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.17 ATP + syntide-2
-
 Homo sapiens ADP + phosphorylated syntide-2
-
 ?
2.7.11.17 additional information components of the Ca2+/calmodulin-dependent protein kinase cascade, overview Homo sapiens ?
-
 ?
2.7.11.17 additional information CaM-K1delta performs autophosphorylation in a Ca2+/calmodulin-dependent manner which has no impact on its kinase activity Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.17 CaM-K1delta
-
 Homo sapiens
2.7.11.17 CaM-KKbeta3
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.17 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.17 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.17 ATP
-
 Homo sapiens