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Literature summary extracted from

  • Yokoyama, R.; Kawasaki, H.; Hirano, H.
    Identification of yeast aspartyl aminopeptidase gene by purifying and characterizing its product from yeast cells (2006), FEBS J., 273, 192-198.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.21 gene YHR113W, located on chromosome VIII, DNA and amino acid sequence determination and analysis Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.21 1,10-phenanthroline 33.5% inhibition at 5 mM Saccharomyces cerevisiae
3.4.11.21 EDTA 76.1% inhibition at 20 mM Saccharomyces cerevisiae
3.4.11.21 additional information poor inhibition by bestatin at 0.2 mM Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.21 0.064
-
 angiotensin I
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.21 56000
-
 monomer, determined by SDS-PAGE Saccharomyces cerevisiae
3.4.11.21 680000
-
 gel filtration Saccharomyces cerevisiae
3.4.11.21 680000
-
 native enzyme, dodecamer, determined using a Superose 6 column Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.21 Saccharomyces cerevisiae
-
-
-
3.4.11.21 Saccharomyces cerevisiae B-8032
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.21 aspartyl aminopeptidase is purified from yeast cells, using ultracentrifugation, ammonium sulfate fractionating, and chromatography on a Bio-Gel column, on a Mono Q column, and a Superose 6 column Saccharomyces cerevisiae
3.4.11.21 native enzyme to homogeneity by ultracentrifugation, ammonium sulfate fractionation, anion exchange chromatography, and gel filtration Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.21 additional information
-
 angiotensin I: 100%, angiotensin II: 144%, angiotensinogen 1-14: 35%, angiotensin II antipeptide: 116%, N-acetyl-angiotensinogen 1-14: 0%, Tyr-bradykinin: 0%, Ile-Ser-bradykinin: 0%, [sar1,ala8] angiotensin II: 0% Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.21 angiotensin I + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.11.21 angiotensin I + H2O
-
 Saccharomyces cerevisiae B-8032 ?
-
 ?
3.4.11.21 angiotensin I + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu Saccharomyces cerevisiae Asp + Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu
-
 ?
3.4.11.21 angiotensin I + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu Saccharomyces cerevisiae B-8032 Asp + Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu
-
 ?
3.4.11.21 angiotensin II + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.11.21 angiotensin II + H2O
-
 Saccharomyces cerevisiae B-8032 ?
-
 ?
3.4.11.21 angiotensin II + H2O i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe, best substrate Saccharomyces cerevisiae Asp + angiotensin III i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe ?
3.4.11.21 angiotensin II + H2O i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe, best substrate Saccharomyces cerevisiae B-8032 Asp + angiotensin III i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe ?
3.4.11.21 angiotensin II antipeptide + H2O i.e. Glu-Gly-Val-Thr-Val-His-Pro-Val Saccharomyces cerevisiae Glu + Gly-Val-Thr-Val-His-Pro-Val
-
 ?
3.4.11.21 angiotensin II antipeptide + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.11.21 angiotensinogen 1-14 + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser, low activity Saccharomyces cerevisiae Asp + Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser
-
 ?
3.4.11.21 angiotensinogen 1-14 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.11.21 additional information the enzyme is specific for unblocked N-terminal acidic amino acid residues, no activity with N-acetyl-angiotensinogen 1-14, Tyr-bradykinin, Ile-Ser-bradykinin, and [Sar1, Ala8]-angiotensin II, overview Saccharomyces cerevisiae ?
-
 ?
3.4.11.21 additional information the enzyme is specific for unblocked N-terminal acidic amino acid residues, no activity with N-acetyl-angiotensinogen 1-14, Tyr-bradykinin, Ile-Ser-bradykinin, and [Sar1, Ala8]-angiotensin II, overview Saccharomyces cerevisiae B-8032 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.21 dodecamer 12 * 56000, SDS-PAGE Saccharomyces cerevisiae
3.4.11.21 dodecamer dodecamer comprising 12 identical units Saccharomyces cerevisiae
3.4.11.21 More peptide mass fingerprinting by MALDI-TOF MS analysis Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.4.11.21 Aminopeptidase
-
 Saccharomyces cerevisiae
3.4.11.21 aspartyl aminopeptidase
-
 Saccharomyces cerevisiae
3.4.11.21 Yhr113w
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.21 37
-
 assay at Saccharomyces cerevisiae
3.4.11.21 37
-
 activity assay Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.21 additional information
-
 neutral pH-optimum Saccharomyces cerevisiae