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Literature summary extracted from

  • Montero, M.; Sanz, L.; Rey, M.; Monte, E.; Llobell, A.
    BGN16.3, a novel acidic beta-1,6-glucanase from mycoparasitic fungus Trichoderma harzianum CECT 2413 (2005), FEBS J., 272, 3441-3448.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.75 additional information BGN16.3 is induced rapidly and strongly by fungal cell walls Trichoderma harzianum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.75 expression profile of BGN16.3 Trichoderma harzianum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.75 extracellular secretion of the beta-1,6-glucanase multienzyme complex, BGN16.3 is part of the complex Trichoderma harzianum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.75 25000 30000 gel filtration Trichoderma harzianum
3.2.1.75 46000
-
 ? * 46000, BGN16.3, SDS-PAGE Trichoderma harzianum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.75 additional information Trichoderma harzianum BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview ?
-
 ?
3.2.1.75 additional information Trichoderma harzianum CECT 2413 BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.75 Trichoderma harzianum
-
 3 isozymes BGN16.1, BGN16.2, and BGN16.3
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3.2.1.75 Trichoderma harzianum CECT 2413
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 3 isozymes BGN16.1, BGN16.2, and BGN16.3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.75 native extracellular BGN16.3 26fold to homogeneity by beta-1,6-glycan affinity chromatography, chromatofocusing, and gel filtration Trichoderma harzianum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.75 additional information expression profile of BGN16.3, regulation of BGN16.3 expression Trichoderma harzianum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.75 188
-
 purified enzyme Trichoderma harzianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.75 glucan + H2O substrate from yeast, 18% of maximal activity Trichoderma harzianum ?
-
 ?
3.2.1.75 glucan + H2O substrate from yeast, 18% of maximal activity Trichoderma harzianum CECT 2413 ?
-
 ?
3.2.1.75 laminarin + H2O 8% of maximal activity Trichoderma harzianum ?
-
 ?
3.2.1.75 laminarin + H2O 8% of maximal activity Trichoderma harzianum CECT 2413 ?
-
 ?
3.2.1.75 additional information BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview Trichoderma harzianum ?
-
 ?
3.2.1.75 additional information substrate specificity, BGN16.3 shows endohydrolytic activity against substrates with beta-1,6-glycosidic linkages, no activity with colloidal chitin, pachyman, starch, cellulose, nigeran, and dextran, no activity with the beta-1,6-disaccharide gentiobiose Trichoderma harzianum ?
-
 ?
3.2.1.75 additional information BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex, regulation of enzyme expression, overview Trichoderma harzianum CECT 2413 ?
-
 ?
3.2.1.75 additional information substrate specificity, BGN16.3 shows endohydrolytic activity against substrates with beta-1,6-glycosidic linkages, no activity with colloidal chitin, pachyman, starch, cellulose, nigeran, and dextran, no activity with the beta-1,6-disaccharide gentiobiose Trichoderma harzianum CECT 2413 ?
-
 ?
3.2.1.75 pustulan + H2O best substrate Trichoderma harzianum oligomers of pustulan product analysis, most abundant products are di-, tri-, and tetra-beta-1,6-glucosides ?
3.2.1.75 pustulan + H2O best substrate Trichoderma harzianum CECT 2413 oligomers of pustulan product analysis, most abundant products are di-, tri-, and tetra-beta-1,6-glucosides ?

Subunits

EC Number Subunits Comment Organism
3.2.1.75 monomer or dimer ? * 46000, BGN16.3, SDS-PAGE Trichoderma harzianum
3.2.1.75 More BGN16.3 is part of the extracellular beta-1,6-glucanase multienzyme complex Trichoderma harzianum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.75 acidic beta-1,6-glucanase
-
 Trichoderma harzianum
3.2.1.75 BGN16.3
-
 Trichoderma harzianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.75 50
-
-
 Trichoderma harzianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.75 5
-
-
 Trichoderma harzianum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.75 4 7 20% of maximal activity at pH 4.0 and pH 7.0 Trichoderma harzianum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.75 Trichoderma harzianum acidic chromatofocusing
-
 4.1
3.2.1.75 Trichoderma harzianum isoelectric focusing
-
 4.5