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Literature summary extracted from

  • Ubhayasekera, W.; Munoz, I.G.; Vasella, A.; Stahlberg, J.; Mowbray, S.L.
    Structures of Phanerochaete chrysosporium Cel7D in complex with product and inhibitors (2005), FEBS J., 272, 1952-1964.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.176 deglycosylated Cel7D complexed with cellobiose, or with inhibitors cellobioimidazole, lactose, or methyl (4S)-beta-cellobiosyl-4-thio-beta-cellobioside, hanging drop vapour diffusion method, 18 mg/ml protein in 10 mM Tris-HCl, pH 7.0, 5 mM CaCl2, 15-22.5% PEG 5000, and 12% glycerol, soaking of crystals in ligand solution containing 10 mM ligand, X-ray diffraction structure determination and analysis at -173°C and 1.7-1.8 A resolution Phanerodontia chrysosporium

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.176 E217Q inactive mutant Phanerodontia chrysosporium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.176 Ca2+
-
 Phanerodontia chrysosporium
3.2.1.176 cellobioimidazole i.e. (5R,6R,7S,8S)-6-(beta-D-glucopyranosyloxy)-5,6,7,8-tetrahydro-5-[(hydroxy)methyl]imidazol[1,2a] pyridine-7,8-diol, the disaccharide binds in the glycosyl-binding subsites +1 and +2 close to the exit of the cellulose-binding tunnel/cleft of Cel7D, binding structure analysis Phanerodontia chrysosporium
3.2.1.176 lactose the disaccharide binds in the glycosyl-binding subsites +1 and +2 close to the exit of the cellulose-binding tunnel/cleft of Cel7D, binding structure analysis Phanerodontia chrysosporium
3.2.1.176 methyl (4S)-beta-cellobiosyl-4-thio-beta-cellobioside a thio-linked substrate analogue, the disaccharide binds in the glycosyl-binding subsites +1 and +2 close to the exit of the cellulose-binding tunnel/cleft of Cel7D, binding structure analysis Phanerodontia chrysosporium
3.2.1.176 Tris
-
 Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.176 additional information
-
 additional information
-
 Phanerodontia chrysosporium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.176 extracellular secretion of Cel7D to the medium Phanerodontia chrysosporium
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.176 cellulose + H2O Phanerodontia chrysosporium
-
 cellobiose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Phanerodontia chrysosporium Q7LIJ0 White Rot fungus, Cel7D is the major cellulase
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.176 glycoprotein Cel7D Phanerodontia chrysosporium

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.176 2 cellohexaose + 2 H2O = 2 cellotriose + cellotetraose + cellobiose substrate binding structure analysis of Cel7D Phanerodontia chrysosporium
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 cellulose + H2O
-
 Phanerodontia chrysosporium cellobiose + ?
-
 ?
3.2.1.176 additional information substrate binding structure analysis of Cel7D Phanerodontia chrysosporium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 Cel7D
-
 Phanerodontia chrysosporium
3.2.1.176 More the enzyme Cel7D belongs to the glycoside hydrolase family 7 Phanerodontia chrysosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.176 30
-
 assay at Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.176 7
-
 assay at Phanerodontia chrysosporium