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Literature summary extracted from

  • Berthonneau, J.; Rodier, M.H.; El Moudni, B.; Jacquemin, J.L.
    Toxoplasma gondii: Purification and characterization of an immunogenic metallopeptidase (2000), Exp. Parasitol., 95, 158-162.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.6 1,10-phenanthroline complete inhibition at 1 mM Toxoplasma gondii
3.4.11.6 DTT 9% inhibition at 1 mM Toxoplasma gondii
3.4.11.6 EDTA 90% inhibition at 1 mM Toxoplasma gondii
3.4.11.6 HgCl2 complete inhibition at 1 mM Toxoplasma gondii
3.4.11.6 iodoacetamide 16% inhibition at 1 mM Toxoplasma gondii
3.4.11.6 leupeptin 5% inhibition at 0.01 mM Toxoplasma gondii
3.4.11.6 additional information no inhibition by phosphoramidon, E64, antipaine, and TLCK Toxoplasma gondii
3.4.11.6 NEM 13% inhibition at 1 mM Toxoplasma gondii
3.4.11.6 pepstatin 5% inhibition at 0.01 mM Toxoplasma gondii
3.4.11.6 PMSF 14% inhibition at 1 mM Toxoplasma gondii
3.4.11.6 ZnCl2 96% inhibition at 1 mM Toxoplasma gondii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.6 soluble
-
 Toxoplasma gondii
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.6 additional information metallopeptidase Toxoplasma gondii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.6 110000
-
 x * 110000, reducing SDS-PAGE Toxoplasma gondii

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.6 Toxoplasma gondii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.6 80.9fold to homogeneity by gel filtration, anion exchange chromatography, and again gel filtration Toxoplasma gondii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.6 26
-
 purified enzyme, substrate N-succinyl-Gly-Pro-Leu-Gly-Pro 7-amido-4-methylcoumarin Toxoplasma gondii
3.4.11.6 55
-
 purified enzyme, substrate L-Leu 7-amido-4-methylcoumarin Toxoplasma gondii
3.4.11.6 140
-
 purified enzyme, substrate L-Tyr 7-amido-4-methylcoumarin Toxoplasma gondii
3.4.11.6 263.7
-
 purified enzyme, substrate L-Arg 7-amido-4-methylcoumarin Toxoplasma gondii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.6 L-Arg 7-amido-4-methylcoumarin + H2O fluorogenic substrate, best substrate Toxoplasma gondii L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Leu 7-amido-4-methylcoumarin + H2O fluorogenic substrate Toxoplasma gondii L-Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Tyr 7-amido-4-methylcoumarin + H2O fluorogenic substrate Toxoplasma gondii L-Tyr + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 N-succinyl-Gly-Pro-Leu-Gly-Pro 7-amido-4-methylcoumarin + H2O fluorogenic substrate Toxoplasma gondii N-succinyl-Gly-Pro-Leu-Gly-Pro + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.6 ? x * 110000, reducing SDS-PAGE Toxoplasma gondii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.6 20
-
 30 min, completely stable Toxoplasma gondii
3.4.11.6 40
-
 30 min, 95% activity remaining Toxoplasma gondii
3.4.11.6 55
-
 30 min, 50% activity remaining Toxoplasma gondii
3.4.11.6 60
-
 30 min, residual activity remaining Toxoplasma gondii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.6 7.4
-
-
 Toxoplasma gondii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.6 6 9 pH profile Toxoplasma gondii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.6 Toxoplasma gondii chromatofocusing
-
 4.7