Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Allegrini, S.; Scaloni, A.; Careddu, M.G.; Cuccu, G.; D'Ambrosio, C.; Pesi, R.; Camici, M.; Ferrara, L.; Tozzi, M.G.
    Mechanistic studies on bovine cytosolic 5'-nucleotidase II, an enzyme belonging to the HAD superfamily (2004), Eur. J. Biochem., 271, 4881-4891.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.5 D351E strong decrease in nucleotidase activity, strong reduction in affinity towards Mg2+ Bos taurus
3.1.3.5 D356E strong decrease in nucleotidase activity, strong reduction in affinity towards Mg2+ Bos taurus
3.1.3.5 D356N strong increase in Km-value for inosine Bos taurus
3.1.3.5 K292M strong decrease in nucleotidase activity Bos taurus
3.1.3.5 K292R strong decrease in nucleotidase activity Bos taurus
3.1.3.5 M53I lowest nucleotidase vs. phosphotransferase activity ratio of all mutants tested Bos taurus
3.1.3.5 M53N strong decrease in catalytic efficiency Bos taurus
3.1.3.5 S251A kinetic behaviour almost similar to wild-type Bos taurus
3.1.3.5 S251T kinetic behaviour almost similar to wild-type Bos taurus
3.1.3.5 T249S kinetic behaviour almost similar to wild-type Bos taurus
3.1.3.5 T249V strongly reduced enzyme activity Bos taurus
3.1.3.5 T56R mutant devoid of phosphotransferase and nucleotidase activities Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 0.1
-
 5'-IMP wild-type Bos taurus
3.1.3.5 0.1
-
 5'-IMP mutant M53N Bos taurus
3.1.3.5 0.1
-
 5'-IMP mutant S251A Bos taurus
3.1.3.5 0.2
-
 5'-IMP mutant D356E Bos taurus
3.1.3.5 0.2
-
 5'-IMP mutant D356N Bos taurus
3.1.3.5 0.2
-
 5'-IMP mutant T249S Bos taurus
3.1.3.5 0.25
-
 Inosine mutant T249v Bos taurus
3.1.3.5 0.3
-
 5'-IMP mutant S251T Bos taurus
3.1.3.5 0.4
-
 5'-IMP mutant T249v Bos taurus
3.1.3.5 0.5
-
 Inosine mutant K292M Bos taurus
3.1.3.5 0.8
-
 Inosine mutant M53I Bos taurus
3.1.3.5 0.9
-
 Inosine mutant D351E Bos taurus
3.1.3.5 0.9
-
 Inosine mutant T249S Bos taurus
3.1.3.5 1
-
 Inosine mutant D356E Bos taurus
3.1.3.5 1
-
 5'-IMP mutant M53I Bos taurus
3.1.3.5 1
-
 Inosine mutant S251A Bos taurus
3.1.3.5 1.1
-
 Inosine wild-type Bos taurus
3.1.3.5 1.1
-
 Inosine mutant M53N Bos taurus
3.1.3.5 1.5
-
 Inosine mutant S251T Bos taurus
3.1.3.5 10.2
-
 Inosine mutant D356N Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 cytosol
-
 Bos taurus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 Mg2+ required Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 60000
-
 x * 60000, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Bos taurus
-
 isoform 5'-nucleotidase II
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.5 a 5'-ribonucleotide + H2O = a ribonucleoside + phosphate conserved motif I is involved in formation and stabilization of the covalent enzyme-phosphate intermediate. T249 and K292 in motif II contribute to stabilize the enzyme-phospho adduct. D351 and D356 in motif III coordinate the magnesium ion required for catalysis Bos taurus
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.3.5 inhibition by CuCl2, 5,5’-dithiobis-(2-nitro-benzoic acid) is reverted by presence of reducing agent Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-IMP + H2O
-
 Bos taurus inosine + phosphate
-
 r
3.1.3.5 inosine + phosphate
-
 Bos taurus 5'-IMP + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
3.1.3.5 ? x * 60000, SDS-PAGE Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.5 0.1
-
 Inosine mutant D351E Bos taurus
3.1.3.5 0.1
-
 Inosine mutant K292M Bos taurus
3.1.3.5 0.3
-
 Inosine mutant T249v Bos taurus
3.1.3.5 0.6
-
 Inosine mutant M53N Bos taurus
3.1.3.5 1.4
-
 Inosine mutant D356N Bos taurus
3.1.3.5 2.2
-
 Inosine mutant D356E Bos taurus
3.1.3.5 3.4
-
 Inosine mutant S251T Bos taurus
3.1.3.5 3.7
-
 Inosine mutant M53I Bos taurus
3.1.3.5 12.3
-
 Inosine mutant S251A Bos taurus
3.1.3.5 19.8
-
 Inosine mutant T249S Bos taurus
3.1.3.5 40
-
 Inosine wild-type Bos taurus