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Literature summary extracted from

  • Pal-Bhowmick, I.; Sadagopan, K.; Vora, H.K.; Sehgal, A.; Sharma, S.; Jarori, G.K.
    Cloning, over-expression, purification and characterization of Plasmodium falciparum enolase (2004), Eur. J. Biochem., 271, 4845-4854.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.11 expression in Escherichia coli strain XL1Blue as an N-terminal His6-tagged protein Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.11 Mg2+ inhibitory at higher concentrations Plasmodium falciparum
4.2.1.11 Na+ 50% inhibition around 0.3-0.4 M Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.11 0.041
-
 (25R)-3beta-hydroxycholest-5-en-27-oate 2-phospho-D-glycerate Plasmodium falciparum
4.2.1.11 0.25
-
 (25R)-3beta-hydroxycholest-5-en-27-oate phosphoenolpyruvate Plasmodium falciparum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.11 K+ slightly activating effect Plasmodium falciparum
4.2.1.11 Mg2+
-
 Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 25710
-
 MALDI-TOF, double charged monomer Plasmodium falciparum
4.2.1.11 50000
-
 SDS-PAGE Plasmodium falciparum
4.2.1.11 51380
-
 MALDI-TOF, single charged monomer Plasmodium falciparum
4.2.1.11 51400
-
 MALDI-TOF Plasmodium falciparum
4.2.1.11 100000
-
 gel filtration Plasmodium falciparum
4.2.1.11 102800
-
 MALDI-TOF, double charged dimer Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate Plasmodium falciparum
-
 phosphoenolpyruvate + H2O
-
 r
4.2.1.11 2-phospho-D-glycerate Plasmodium falciparum NF54
-
 phosphoenolpyruvate + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Plasmodium falciparum
-
-
-
4.2.1.11 Plasmodium falciparum NF54
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 Ni-nitrilotriacetic acid affinity chromatography, eluted with imidazole Plasmodium falciparum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.11 30
-
 2-phospho-D-glycerate, pH 7.4, 20°C, Tris/HCl Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 (25R)-3beta-hydroxycholest-5-en-27-oate
-
 Plasmodium falciparum ?
-
 ?
4.2.1.11 (25R)-3beta-hydroxycholest-5-en-27-oate
-
 Plasmodium falciparum NF54 ?
-
 ?
4.2.1.11 2-phospho-D-glycerate
-
 Plasmodium falciparum phosphoenolpyruvate + H2O
-
 r
4.2.1.11 2-phospho-D-glycerate
-
 Plasmodium falciparum NF54 phosphoenolpyruvate + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.11 homodimer 2 * 50000, gel filtration Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 r-Pfen
-
 Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.11 7.4 7.6 irrespective of substrate Plasmodium falciparum