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Literature summary extracted from

  • Yamamoto, K.; Nakayama, A.; Yamamoto, Y.; Tabata, S.
    Val216 decides the substrate specificity of alpha-glucosidase in Saccharomyces cerevisiae (2004), Eur. J. Biochem., 271, 3414-3420.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.20 cloning of the maltase gene from strain 727-14C, cloning from a cDNA library and amino acid sequence determination, expression of wild-type enzyme in Escherichia coli strain KP3998, expression of chimeric maltase/isomaltase mutant enzymes in Escherichia coli strain KP3998 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.20 additional information construction of chimeric maltase/isomaltase mutant enzymes, mutants D215A, V216T, G217A, and S218G of isomaltase are constructed mimicking the maltase sequence, isomaltase mutant V216T shows the same substrate specificity for maltose and isomaltose, while the wild-type isomaltase is not active with maltose and the wild-type maltase is not active with isomaltose, overview Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 0.31
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 30°C, recombinant wild-type enzyme Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.20 maltose + H2O Saccharomyces cerevisiae
-
 alpha-D-glucose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Saccharomyces cerevisiae
-
 strains D-346, ATCC 56960, and 727-14C, ATCC 56959
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 recombinant enzyme from Escherichia coli by ion exchange and hydroxyapatite chromatography Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.20 maltotetraose + 3 H2O = 4 alpha-D-glucose Val216 determines the substrate specificity of the yeast enzyme, active site structure modelling Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 70
-
 purified recombinant wild-type enzyme, substrate maltose Saccharomyces cerevisiae
3.2.1.20 132
-
 purified recombinant wild-type enzyme, substrate 4-nitrophenyl alpha-D-glucoside Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Saccharomyces cerevisiae 4-nitrophenol + alpha-D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Saccharomyces cerevisiae alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 additional information no activity with isomaltose, Val216 decides the enzyme's substrate specificity Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 maltase
-
 Saccharomyces cerevisiae