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Literature summary extracted from

  • Masson, P.; Bec, N.; Froment, M.T.; Nachon, F.; Balny, C.; Lockridge, O.; Schopfer, L.M.
    Rate-determining step of butyrylcholinesterase-catalyzed hydrolysis of benzoylcholine and benzoylthiocholine. Volumetric study of wild-type and D70G mutant behavior (2004), Eur. J. Biochem., 271, 1980-1990.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.8 D70G 7-20fold increase in Km-value Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.8 0.00295
-
 benzoylthiocholine wild-type, pH 7.4, 25°C Homo sapiens
3.1.1.8 0.00301
-
 Benzoylcholine wild-type, pH 7.4, 25°C Homo sapiens
3.1.1.8 0.0211
-
 Benzoylcholine mutant D70G, pH 7.4, 25°C Homo sapiens
3.1.1.8 0.0592
-
 benzoylthiocholine mutant D70G, pH 7.4, 25°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.8 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.8 benzoylcholine + H2O rate-limiting step for hydrolysis is deacylation, k3 Homo sapiens choline + benzoate
-
 ?
3.1.1.8 benzoylthiocholine + H2O rate-limiting step for hydrolysis is acylation, k2 Homo sapiens thiocholine + benzoate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.8 0.82
-
 benzoylthiocholine wild-type, pH 7.4, 25°C Homo sapiens
3.1.1.8 1.3
-
 benzoylthiocholine mutant D70G, pH 7.4, 25°C Homo sapiens
3.1.1.8 14.7
-
 Benzoylcholine wild-type, pH 7.4, 25°C Homo sapiens
3.1.1.8 15
-
 Benzoylcholine mutant D70G, pH 7.4, 25°C Homo sapiens