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Literature summary extracted from

  • Martins, A.S.; Peixoto, D.; Paiva, L.M.C.; Panek, A.D.; Paiva, C.L.A.
    A simple method for obtaining reusable reactors containing immobilized trehalase: characterization of a crude trehalase preparation immobilized on chitin particles (2006), Enzyme Microb. Technol., 38, 486-492.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.28 biotechnology immobilization of the crude enzyme on chitin resulting in stable, specific, and reusable reactors for application in other biotechnological processes, the immobilized enzyme shows no significant loss of activity being reused 10times and stored at 10°C, in 50 mM sodium maleate, pH 6.0, for 55 days Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.28 overexpression of the enzyme in strain Mph2 from plasmid pTRE11 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 0.5
-
 alpha,alpha-trehalose pH 5.5, 30°C, immobilized enzyme Escherichia coli
3.2.1.28 1.32
-
 alpha,alpha-trehalose pH 5.5, 30°C, soluble enzyme Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.28 periplasm
-
 Escherichia coli
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 58000
-
 x * 58000, recombinant enzyme Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Escherichia coli
-
-
-
3.2.1.28 Escherichia coli Mph2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28 preparation of periplasmic fraction of recombinant Escherichia coli Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.2.1.28 immobilized crude recombinant enzyme on chitin shows no significant loss of activity being reused 10times and stored at 10°C, in 50 mM sodium maleate, pH 6.0, for 55 days Escherichia coli

Subunits

EC Number Subunits Comment Organism
3.2.1.28 ? x * 58000, recombinant enzyme Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.2.1.28 trehalase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.28 50
-
 immobilized enzyme Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 5.5
-
 immobilized enzyme Escherichia coli