Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Harvey, S.P.; Kolakowski, J.E.; Cheng, T.C.; Rastogi, V.K.; Reiff, L.P.; DeFrank, J.J.; Raushel, F.M.; Hill, C.
    Stereospecificity in the enzymatic hydrolysis of cyclosarin (GF) (2005), Enzyme Microb. Technol., 37, 547-555.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.8.2 expression in Escherichia coli Alteromonas sp.
3.1.8.2 expression in Escherichia coli Pseudoalteromonas haloplanktis

Organism

EC Number Organism UniProt Comment Textmining
3.1.8.2 Alteromonas sp.
-
 JD6.5
-
3.1.8.2 Alteromonas sp. JD6.5
-
 JD6.5
-
3.1.8.2 Pseudoalteromonas haloplanktis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.8.2
-
 Alteromonas sp.
3.1.8.2
-
 Pseudoalteromonas haloplanktis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.8.2 2.4
-
 O-cyclohexyl methylphosphonofluoridate (-)isomer as substrate, pH 7.2, 1 mM MnCl2 Alteromonas sp.
3.1.8.2 2.7
-
 O-cyclohexyl methylphosphonofluoridate (-)isomer as substrate, pH 7.2, 1 mM MnCl2 Pseudoalteromonas haloplanktis
3.1.8.2 29.2
-
 O-cyclohexyl methylphosphonofluoridate (+)isomer as substrate, pH 7.2, 1 mM MnCl2 Alteromonas sp.
3.1.8.2 65.3
-
 O-cyclohexyl methylphosphonofluoridate (+)isomer as substrate, pH 7.2, 1 mM MnCl2 Pseudoalteromonas haloplanktis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.8.2 cyclohexyl methyl fluorophosphate + H2O (+)isomer, pH 7.2, 1 mM MnCl2 Alteromonas sp. cyclohexyl methyl hydrogen phosphate + fluoride
-
 ?
3.1.8.2 cyclohexyl methyl fluorophosphate + H2O (+)isomer, pH 7.2, 1 mM MnCl2 Pseudoalteromonas haloplanktis cyclohexyl methyl hydrogen phosphate + fluoride
-
 ?
3.1.8.2 cyclohexyl methyl fluorophosphate + H2O (+)isomer, pH 7.2, 1 mM MnCl2 Alteromonas sp. JD6.5 cyclohexyl methyl hydrogen phosphate + fluoride
-
 ?
3.1.8.2 O-cyclohexyl methylphosphonofluoridate + H2O no reaction with the (-)isomer O-cyclohexyl methylphosphonofluoridate Alteromonas sp. O-cyclohexyl methylphosphonate + fluoride
-
 ?
3.1.8.2 O-cyclohexyl methylphosphonofluoridate + H2O no reaction with the (-)isomer O-cyclohexyl methylphosphonofluoridate Pseudoalteromonas haloplanktis O-cyclohexyl methylphosphonate + fluoride
-
 ?
3.1.8.2 O-cyclohexyl methylphosphonofluoridate + H2O no reaction with the (-)isomer O-cyclohexyl methylphosphonofluoridate Alteromonas sp. JD6.5 O-cyclohexyl methylphosphonate + fluoride
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.8.2 OPAA
-
 Alteromonas sp.
3.1.8.2 OPAA
-
 Pseudoalteromonas haloplanktis
3.1.8.2 organophosphorus acid anhydrolase
-
 Alteromonas sp.
3.1.8.2 organophosphorus acid anhydrolase
-
 Pseudoalteromonas haloplanktis