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Literature summary extracted from

  • Ben Bacha, A.; Gargouri, Y.; Ben Ali, Y.; Miled, N.; Reinbolt, J.; Mejdoub, H.
    Purification and biochemical characterization of ostrich pancreatic lipase (2005), Enzyme Microb. Technol., 37, 309-317.
No PubMed abstract available

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 additional information the enzyme possesses two free SH groups Struthio camelus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 45000
-
 gel filtration Struthio camelus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Struthio camelus
-
 ostrich
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.3 no glycoprotein
-
 Struthio camelus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 native enzyme 13.4fold from pancreas by ammonium sulfate fractionation, two steps of gel filtration, and cation exchange chromatography Struthio camelus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 pancreas
-
 Struthio camelus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 6000
-
 purified enzyme, titrimetric assay method, substrate tributyrin Struthio camelus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 liprocil + H2O
-
 Struthio camelus ?
-
 ?
3.1.1.3 additional information the pancreatic enzyme shows a wide substrate spectrum but prefers triacylglyceride substrates with short fatty acid chains Struthio camelus ?
-
 ?
3.1.1.3 tributyrin + H2O preferred substrate Struthio camelus dibutyrin + butyrate
-
 ?
3.1.1.3 tripropionin + H2O
-
 Struthio camelus dipropionin + propanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 monomer 1 * 45000, SDS-PAGE Struthio camelus
3.1.1.3 More peptide mapping Struthio camelus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Struthio camelus
3.1.1.3 OPL
-
 Struthio camelus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 37
-
 assay at Struthio camelus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8.5
-
 assay at Struthio camelus