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Literature summary extracted from

  • Yoon, J.H.; Mckenzie, D.
    A comparison of the activities of three beta-galactosidases in aqueous-organic solvent mixtures (2005), Enzyme Microb. Technol., 36, 439-446.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 o-nitrophenyl-beta-D-galactoside
-
 Aspergillus oryzae
3.2.1.23 o-nitrophenyl-beta-D-galactoside
-
 Escherichia coli
3.2.1.23 o-nitrophenyl-beta-D-galactoside
-
 Kluyveromyces marxianus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.23 2-methyoxyethyl acetate 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 70% loss of activity Escherichia coli
3.2.1.23 2-methyoxyethyl acetate 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 70% loss of activity Aspergillus oryzae
3.2.1.23 Acetone 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 60% loss of activity Escherichia coli
3.2.1.23 Acetone 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 75% loss of activity Aspergillus oryzae
3.2.1.23 acetonitrile 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 60% loss of activity Escherichia coli
3.2.1.23 acetonitrile 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 70% loss of activity Aspergillus oryzae
3.2.1.23 diethyleneglycol diethyl ether 37°C, 50% v/v organic solvent-buffer mixture, 5 min,about 60% loss of activity Escherichia coli
3.2.1.23 diethyleneglycol diethyl ether 37°C, 50% v/v organic solvent-buffer mixture, 5 min,about 90% loss of activity Aspergillus oryzae
3.2.1.23 diglyme 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 60% loss of activity Escherichia coli
3.2.1.23 diglyme 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 80% loss of activity Aspergillus oryzae
3.2.1.23 monoglyme 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 50% loss of activity Escherichia coli
3.2.1.23 monoglyme 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 75% loss of activity Aspergillus oryzae
3.2.1.23 tetraglyme 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 40% loss of activity Escherichia coli
3.2.1.23 tetraglyme 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 75% loss of activity Aspergillus oryzae
3.2.1.23 triethyl phosphate 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 40% loss of activity Escherichia coli
3.2.1.23 triethyl phosphate 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 75% loss of activity Aspergillus oryzae
3.2.1.23 triglyme 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 75% loss of activity Escherichia coli
3.2.1.23 triglyme 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 80% loss of activity Aspergillus oryzae
3.2.1.23 trimethyl phosphate 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 60% loss of activity Escherichia coli
3.2.1.23 trimethyl phosphate 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 75% loss of activity Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Aspergillus oryzae
-
-
-
3.2.1.23 Escherichia coli
-
-
-
3.2.1.23 Kluyveromyces marxianus
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.2.1.23 beta-galactosidase activity in 80% v/v buffer tetraglyme after 11 h is 9% of the initial activity of the enzyme in 50 mM imidazole/HCl buffer containing 1 mM MgCl2, 145 mM NaCl and 4 mM dithiothreitol at pH 7.0. The recovered activity of the enzyme, measured after dialysis is approximately 45% of the initial activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O no hydrolysis activity is observed in 1,4-dioxane, methylsulphoxide, N,N-dimethylformamide, or pyridine Escherichia coli o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O no hydrolysis activity is observed in 1,4-dioxane, methylsulphoxide, N,N-dimethylformamide, or pyridine Kluyveromyces marxianus o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O no hydrolysis activity is observed in 1,4-dioxane, methylsulphoxide, N,N-dimethylformamide, or pyridine Aspergillus oryzae o-nitrophenol + beta-D-galactose
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.23 0.23
-
 o-nitrophenyl-beta-D-galactoside 30°C, in 50 mM imidazole/HCl buffer containing 1 mM MgCl2, 145 mM NaCl and 4 mM dithiothreitol at pH 6.5 Kluyveromyces marxianus
3.2.1.23 0.28
-
 o-nitrophenyl-beta-D-galactoside 37°C, in 50 mM sodium acetate/acetic acid buffer at pH 4.5 Aspergillus oryzae
3.2.1.23 0.3
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: 2-methyoxyethyl acetate, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
3.2.1.23 0.3
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: acetone, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
3.2.1.23 0.3
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: monoglyme, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
3.2.1.23 0.3
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: triethyl phosphate, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
3.2.1.23 0.3
-
 o-nitrophenyl-beta-D-galactoside 37°C, in 50 mM imidazole/HCl buffer containing 1 mM MgCl2, 145 mM NaCl and 4 mM dithiothreitol at pH 7.0 Escherichia coli
3.2.1.23 0.35
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: triglyme, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
3.2.1.23 0.4
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: 2-methyoxyethyl acetate, 50% v/v organic solvent-buffer mixture Escherichia coli
3.2.1.23 0.42
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: acetonitrile, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
3.2.1.23 0.58
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: diglyme, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
3.2.1.23 0.59
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: trimethyl phosphate, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
3.2.1.23 0.61
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: acetone, 50% v/v organic solvent-buffer mixture Escherichia coli
3.2.1.23 0.81
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: acetonitrile, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
3.2.1.23 0.83
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: trimethyl phosphate, 50% v/v organic solvent-buffer mixture Escherichia coli
3.2.1.23 0.87
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: tetraglyme, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
3.2.1.23 0.97
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: diethyleneglycol diethyl ether, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
3.2.1.23 0.99
-
 o-nitrophenyl-beta-D-galactoside 30°C, solvent: tetraglyme, 50% v/v organic solvent-buffer mixture Kluyveromyces marxianus
3.2.1.23 1.04
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: monoglyme, 50% v/v organic solvent-buffer mixture Escherichia coli
3.2.1.23 1.09
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: diglyme, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
3.2.1.23 1.21
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: acetonitrile, 50% v/v organic solvent-buffer mixture Escherichia coli
3.2.1.23 1.38
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: acetone, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
3.2.1.23 1.4
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: trimethyl phosphate, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
3.2.1.23 1.44
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: diethyleneglycol diethyl ether, 50% v/v organic solvent-buffer mixture Escherichia coli
3.2.1.23 1.55
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: diethyleneglycol diethyl ether, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
3.2.1.23 1.64
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: triglyme, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
3.2.1.23 1.67
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: tetraglyme, 50% v/v organic solvent-buffer mixture Escherichia coli
3.2.1.23 1.71
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: diglyme, 50% v/v organic solvent-buffer mixture Escherichia coli
3.2.1.23 1.76
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: monoglyme, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
3.2.1.23 1.84
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: triethyl phosphate, 50% v/v organic solvent-buffer mixture Escherichia coli
3.2.1.23 2.07
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: 2-methyoxyethyl acetate, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
3.2.1.23 2.92
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: triethyl phosphate, 50% v/v organic solvent-buffer mixture Aspergillus oryzae
3.2.1.23 3.38
-
 o-nitrophenyl-beta-D-galactoside 37°C, solvent: triglyme, 50% v/v organic solvent-buffer mixture Escherichia coli