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Literature summary extracted from
- Purification and properties of a maltotriose-producing alpha-amylase from Thermobifida fusca (2004), Enzyme Microb. Technol., 35, 254-260.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.116 | Hg2+ | 1 mM | Thermobifida fusca |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.116 | additional information | - |
additional information | Km-value for soluble starch is 0.88 mg/ml | Thermobifida fusca |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.116 | 60000 | - |
gel filtration | Thermobifida fusca |
| 3.2.1.116 | 64000 | - |
1 * 64000, SDS-PAGE | Thermobifida fusca |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.116 | Thermobifida fusca | - |
- |
- |
| 3.2.1.116 | Thermobifida fusca NTU22 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.116 | - |
Thermobifida fusca |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.116 | raw sago starch + H2O | - |
Thermobifida fusca | maltotriose + ? | - |
? | |
| 3.2.1.116 | raw sago starch + H2O | - |
Thermobifida fusca NTU22 | maltotriose + ? | - |
? | |
| 3.2.1.116 | soluble starch + H2O | - |
Thermobifida fusca | maltotriose + ? | - |
? | |
| 3.2.1.116 | soluble starch + H2O | - |
Thermobifida fusca NTU22 | maltotriose + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.116 | monomer | 1 * 64000, SDS-PAGE | Thermobifida fusca |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.116 | maltotriose-producing alpha-amylase | - |
Thermobifida fusca |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.116 | 60 | - |
- |
Thermobifida fusca |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.116 | 60 | - |
3 h, about 70% of the activity remains | Thermobifida fusca |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.116 | 7 | - |
- |
Thermobifida fusca |
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Release 2023.1 (January 2023)
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