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Literature summary extracted from

  • Yang, J.; Roe, S.M.; Cliff, M.J.; Williams, M.A.; Ladbury, J.E.; Cohen, P.T.; Barford, D.
    Molecular basis for TPR domain-mediated regulation of protein phosphatase 5 (2005), EMBO J., 24, 1-10.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.16 arachidonic acid activation mechanism by prompting an alternate conformation of the TPR domain, destabilising the TPR domain-enzyme interface Homo sapiens
3.1.3.16 Hsp90 heat shock protein, activation mechanism via interruption of the TPR domain interactions Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.16 expression of Ppp5 in Escherichia coli strain B834 Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.16 purified recombinant enzyme, under-oil-batch method, 21 mg/ml protein in solution is mixed with an eqal volume of crystallization buffer containing 10% w/v PEG 6000, 4% 2-methyl-2,4-pentanediol, 0.1 M Na-HEPES, pH 8.5, 3 mM DTT, under silicon oil at 14°C, cryoprotection buffer contains 13% w/v PEG 6000, 18% 2-methyl-2,4-pentanediol, 0.075 M NaHEPES, pH 8.5, X-ray diffraction structure determination and analysis at 2.9 A resolution, molecular replacement, modeling Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.16 additional information Ppp5 is inhibited by an autoinhitory conformation formed by the N-terminal TPR domain and a C-terminal subdomain, structure and mechanism overview Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.16 Mn2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.16 additional information Homo sapiens Ppp5 is involved in signalling pathways that control cellular responses to stress, glucocorticoids, and DNA damage, molecular basis for N-terminal tetratricopeptide repeat domain TPR-mediated regulation of Ppp5 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.16 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.16 recombinant Ppp5 from Escherichia coli strain B834 by cation exchange, hydrophobic interaction, and nickel affinity chromatography and gel filtration Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.16 [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate Glu76 is involved in catalysis, active site structure Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.16 additional information Ppp5 is involved in signalling pathways that control cellular responses to stress, glucocorticoids, and DNA damage, molecular basis for N-terminal tetratricopeptide repeat domain TPR-mediated regulation of Ppp5 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.16 More molecular basis for N-terminal TPR domain-mediated regulation of Ppp5, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.3.16 Ppp5
-
 Homo sapiens
3.1.3.16 protein phosphatase 5
-
 Homo sapiens
3.1.3.16 serine/threonine phosphatase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.16 22
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.16 8
-
 assay at Homo sapiens