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Literature summary extracted from

  • Ohta, Y.; Hatada, Y.; Miyazaki, M.; Nogi, Y.; Ito, S.; Horikoshi, K.
    Purification and characterization of a novel alpha-agarase from a Thalassomonas sp. (2005), Curr. Microbiol., 50, 212-216.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.158
-
 Thalassomonas sp.

General Stability

EC Number General Stability Organism
3.2.1.158 Ca2+ stabilizes enzyme Thalassomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.158 extracellular using Bacillus subtilis as the host Thalassomonas sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.158 Ca2+ required, about 15% residual activity in absence of Ca2+ Thalassomonas sp.
3.2.1.158 Ca2+ stabilizes enzyme Thalassomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.158 85000
-
 gel filtration Thalassomonas sp.
3.2.1.158 85000
-
 SDS-PAGE and gel filtration Thalassomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.158 Thalassomonas sp.
-
-
-
3.2.1.158 Thalassomonas sp. JAMB-A33
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.158
-
 Thalassomonas sp.
3.2.1.158 isolated from the sediment of Noma point, Japan, at a depth of 230m, purified by DEAE-Toyopearl 650M column, ultracentrifugation and gel filtration Thalassomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.158 additional information isolated from the sediment of Noma point, Japan, at a depth of 230m Thalassomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.158 40.7
-
-
 Thalassomonas sp.
3.2.1.158 40.7
-
 pH 8.5, 45°C Thalassomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.158 agarohexaose + H2O
-
 Thalassomonas sp. agarotetraose + agarobiose
-
 ?
3.2.1.158 agarohexaose + H2O
-
 Thalassomonas sp. JAMB-A33 agarotetraose + agarobiose
-
 ?
3.2.1.158 agarohexaose + H2O
-
 Thalassomonas sp. ?
-
 ?
3.2.1.158 agarose + H2O
-
 Thalassomonas sp. agarotetraose + ?
-
 ?
3.2.1.158 agarose + H2O
-
 Thalassomonas sp. JAMB-A33 agarotetraose + ?
-
 ?
3.2.1.158 additional information no substrate: kappa-, or lambda-carrageenans, p-nitrophenyl-galactopyranosides Thalassomonas sp. ?
-
 ?
3.2.1.158 additional information no substrate: kappa-, or lambda-carrageenans, p-nitrophenyl-galactopyranosides Thalassomonas sp. JAMB-A33 ?
-
 ?
3.2.1.158 neoagarohexaose + H2O
-
 Thalassomonas sp. agarotetraose
-
 ?
3.2.1.158 neoagarohexaose + H2O
-
 Thalassomonas sp. JAMB-A33 agarotetraose
-
 ?
3.2.1.158 neoagarohexaose + H2O
-
 Thalassomonas sp. ?
-
 ?
3.2.1.158 porphyran + H2O
-
 Thalassomonas sp. ?
-
 ?
3.2.1.158 porphyran + H2O at 95% of the rate with agarose Thalassomonas sp. ?
-
 ?
3.2.1.158 porphyran + H2O
-
 Thalassomonas sp. JAMB-A33 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.158 monomer 1 * 85000, SDS-PAGE Thalassomonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.158 agaraseA33
-
 Thalassomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.158 45
-
-
 Thalassomonas sp.
3.2.1.158 45
-
 50 mM N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid-NaOH buffer, pH 8.5, containing 5 mM CaCl2 Thalassomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.158 40
-
 stable for at least 30 min Thalassomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.158 8.5
-
-
 Thalassomonas sp.
3.2.1.158 8.5
-
 25°C, 30 min, different buffers Thalassomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.158 4.5 9.5
-
 Thalassomonas sp.
3.2.1.158 6 11
-
 Thalassomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.158 6 11
-
 Thalassomonas sp.