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Literature summary extracted from

  • Decelle, B.; Tsang, A.; Storms, R.K.
    Cloning, functional expression and characterization of three Phanerochaete chrysosporium endo-1,4-beta-xylanases (2004), Curr. Genet., 46, 166-175.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Hg2+ 2 mM, 20% residual activity for isoforms xynA, xynC, 67% residual activity for isoform xynB Phanerodontia chrysosporium
3.2.1.8 Pb2+ 2 mM, 70% residual activity for isoforms xynA, xynC, 80% residual activity for isoform xynB Phanerodontia chrysosporium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 28600
-
 x * 41600, isoform xynA, x * 28600, isoform xynB, x * 40300, isoform xynC, calculated, x * 52000, isoform xynA, x * 50000, isoform xynB, x * 32000, isoform xynC, SDS-PAGE Phanerodontia chrysosporium
3.2.1.8 32000
-
 x * 41600, isoform xynA, x * 28600, isoform xynB, x * 40300, isoform xynC, calculated, x * 52000, isoform xynA, x * 50000, isoform xynB, x * 32000, isoform xynC, SDS-PAGE Phanerodontia chrysosporium
3.2.1.8 40300
-
 x * 41600, isoform xynA, x * 28600, isoform xynB, x * 40300, isoform xynC, calculated, x * 52000, isoform xynA, x * 50000, isoform xynB, x * 32000, isoform xynC, SDS-PAGE Phanerodontia chrysosporium
3.2.1.8 41600
-
 x * 41600, isoform xynA, x * 28600, isoform xynB, x * 40300, isoform xynC, calculated, x * 52000, isoform xynA, x * 50000, isoform xynB, x * 32000, isoform xynC, SDS-PAGE Phanerodontia chrysosporium
3.2.1.8 50000
-
 x * 41600, isoform xynA, x * 28600, isoform xynB, x * 40300, isoform xynC, calculated, x * 52000, isoform xynA, x * 50000, isoform xynB, x * 32000, isoform xynC, SDS-PAGE Phanerodontia chrysosporium
3.2.1.8 52000
-
 x * 41600, isoform xynA, x * 28600, isoform xynB, x * 40300, isoform xynC, calculated, x * 52000, isoform xynA, x * 50000, isoform xynB, x * 32000, isoform xynC, SDS-PAGE Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Phanerodontia chrysosporium
-
 isoforms xynA, xynC, family 10 glycoside hydrolases, xynB, family 11 glycoside hydrolase
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.8 additional information sequences contain several potential O-glycosylation sites, sequence of xynB contains addtitionally a potential N-glycosylation site Phanerodontia chrysosporium

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 after expression in Aspergillus niger Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 p-nitrophenyl beta-D-cellobioside + H2O weak substrate for isoforms xynA, xynC, no substrate for xynB Phanerodontia chrysosporium ?
-
 ?
3.2.1.8 p-nitrophenyl beta-D-xylopyranoside + H2O weak substrate for isoforms xynA, xynC, no substrate for xynB Phanerodontia chrysosporium p-nitrophenol + beta-D-xylopyranoside
-
 ?
3.2.1.8 xylan + H2O from birchwood, beechwood or oatspelt. Oatspelt xylan is slightly preferred Phanerodontia chrysosporium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 41600, isoform xynA, x * 28600, isoform xynB, x * 40300, isoform xynC, calculated, x * 52000, isoform xynA, x * 50000, isoform xynB, x * 32000, isoform xynC, SDS-PAGE Phanerodontia chrysosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 60
-
 isoform xynB Phanerodontia chrysosporium
3.2.1.8 70
-
 isoforms xynA, xynC Phanerodontia chrysosporium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 50
-
 isoform xynB, stable up to Phanerodontia chrysosporium
3.2.1.8 60
-
 isoforms xynA, xynC, stable for 30 min Phanerodontia chrysosporium
3.2.1.8 70
-
 isoforms xynA, xynB, xynC, complete inactivation in 30 min Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 4.5
-
 isoforms xynA, xynB, xynC Phanerodontia chrysosporium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 3 8 isoforms xynA, xynB, stable for at least 2 h Phanerodontia chrysosporium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Phanerodontia chrysosporium isoform xynC, calculated
-
 5.5
3.2.1.8 Phanerodontia chrysosporium isoform xynA, calculated
-
 5.8
3.2.1.8 Phanerodontia chrysosporium isoform xynB, calculated
-
 6