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Literature summary extracted from

  • Jing, G.; Li, L.; Li, Y.; Xie, L.; Zhang, R.
    Purification and partial characterization of two acid phosphatase forms from pearl oyster (Pinctada fucata) (2006), Comp. Biochem. Physiol. B, 143, 229-235.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.2 Co2+ 311% activity of isozyme II, no effect on isozyme I Pinctada fucata
3.1.3.2 Cr3+ 130% activity of isozyme I, no effect on isozyme II Pinctada fucata
3.1.3.2 Fe2+ 152% activity of isozyme II Pinctada fucata
3.1.3.2 Mn2+ 113% activity of isozyme II, 107% of isozyme I Pinctada fucata

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 Cu2+ 92% inhibition of isozyme I and 45% inhibition of isozyme II at 5 mM Pinctada fucata
3.1.3.2 EDTA 21% inhibition of isozyme I and poor inhibition of isozyme II at 5 mM Pinctada fucata
3.1.3.2 Fe2+ 30% inhibition of isozyme I at 5 mM Pinctada fucata
3.1.3.2 Fe3+ strong inhibition of both isozymes at 5 mM Pinctada fucata
3.1.3.2 fluoride complete inhibition of both isozymes at 5 mM Pinctada fucata
3.1.3.2 L-Tartrate 95% inhibition of isozyme I and 77% inhibition of isozyme II at 20 mM Pinctada fucata
3.1.3.2 additional information no or poor inhibition of both isozymes by EGTA and Cd2+ Pinctada fucata
3.1.3.2 Pb2+ 54% inhibition of isozyme I and 41% inhibition of isozyme II at 5 mM Pinctada fucata
3.1.3.2 tungstate over 90% inhibition of both isozymes at 5 mM Pinctada fucata
3.1.3.2 Zn2+ 56% inhibition of isozyme I and 27% inhibition of isozyme II at 5 mM Pinctada fucata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.194
-
 4-nitrophenyl phosphate pH 4.6, 47°C, purified isozyme I Pinctada fucata
3.1.3.2 0.237
-
 4-nitrophenyl phosphate pH 3.2, 57°C, purified isozyme II Pinctada fucata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 29800
-
 2 * 29800, isozyme II, SDS-PAGE Pinctada fucata
3.1.3.2 197100
-
 isozyme I, gel filtration Pinctada fucata
3.1.3.2 208800
-
 1 * 208800, isozyme I, SDS-PAGE Pinctada fucata

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Pinctada fucata
-
 pearl oyster, two isozymes AcPAse I and II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 isozymes AcPAse I and II, 160.5fold and 613.6fold, respectively, from viscera to homogeneity by DEAE ion exchange chromatography, gel filtration, and concanavalin A affinity chromatography Pinctada fucata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 viscus
-
 Pinctada fucata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 0.58
-
 purified isozyme I Pinctada fucata
3.1.3.2 0.73
-
 purified isozyme II Pinctada fucata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 3-phosphoglycerate + H2O
-
 Pinctada fucata glycerate + phosphate
-
 ?
3.1.3.2 4-nitrophenyl phosphate + H2O best substrate Pinctada fucata 4-nitrophenol + phosphate
-
 ?
3.1.3.2 ADP + H2O
-
 Pinctada fucata AMP + phosphate
-
 ?
3.1.3.2 alpha-naphthyl phosphate + H2O
-
 Pinctada fucata alpha-naphthol + phosphate
-
 ?
3.1.3.2 AMP + H2O best substrate Pinctada fucata adenosine + phosphate
-
 ?
3.1.3.2 ATP + H2O
-
 Pinctada fucata ADP + phosphate
-
 ?
3.1.3.2 D-fructose 6-phosphate + H2O
-
 Pinctada fucata D-fructose + phosphate
-
 ?
3.1.3.2 D-glucose 1-phosphate + H2O
-
 Pinctada fucata D-glucose + phosphate
-
 ?
3.1.3.2 D-glucose 6-phosphate + H2O
-
 Pinctada fucata D-glucose + phosphate
-
 ?
3.1.3.2 additional information isozymes I and II are non-specific and show a broad substrate spectrum, substrate specificties, overview Pinctada fucata ?
-
 ?
3.1.3.2 phenyl phosphate + H2O
-
 Pinctada fucata phenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 dimer 2 * 29800, isozyme II, SDS-PAGE Pinctada fucata
3.1.3.2 monomer 1 * 208800, isozyme I, SDS-PAGE Pinctada fucata

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 AcPase
-
 Pinctada fucata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 47
-
 isozyme I Pinctada fucata
3.1.3.2 57
-
 isozyme II Pinctada fucata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 50
-
 isozymes I and II are stable for 40 min Pinctada fucata
3.1.3.2 60
-
 isozyme I shows 81% reduction of activity after 5 min, isozyme II shows 57% reduction of activity after 40 min Pinctada fucata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 3.2
-
 isozyme II Pinctada fucata
3.1.3.2 4.6
-
 isozyme I Pinctada fucata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.2 2.8 4.4 isozyme II, 80% activity at pH 2.8 and pH 4.4 Pinctada fucata
3.1.3.2 4 5.2 isozyme I, 80% activity at pH 4.0 and pH 5.2 Pinctada fucata