Any feedback?
Literature summary extracted from
- Two forms of alpha-amylase in mantle tissue of Mytilus galloprovincialis: purification and molecular properties of form II (2005), Comp. Biochem. Physiol. B, 142, 56-66.
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | Ca2+ | activates, activity profile | Mytilus galloprovincialis |  |
| 3.2.1.1 | additional information | the enzyme is not affected by Mn2+ at 1-100 mM | Mytilus galloprovincialis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
enzyme forms I and II, gel filtration, overview | Mytilus galloprovincialis |
| 3.2.1.1 | 66000 | - |
x * 66000, enzyme form II, SDS-PAGE | Mytilus galloprovincialis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Mytilus galloprovincialis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | native enzyme from mantle tissue by insoluble starch affinity chromatography and gel filtration, resolution of two enzyme forms, an aggregated form I and a monomeric form II, 142.4fold and 26.5fold purification, respectively | Mytilus galloprovincialis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | mantle | - |
Mytilus galloprovincialis | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 8 | - |
purified enzyme form II | Mytilus galloprovincialis |
| 3.2.1.1 | 42 | - |
purified enzyme form I | Mytilus galloprovincialis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | glycogen + H2O | - |
Mytilus galloprovincialis | maltose + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | monomer | x * 66000, enzyme form II, SDS-PAGE | Mytilus galloprovincialis |
| 3.2.1.1 | More | the enzyme occurs in two forms, an aggregated form I and a monomeric form II, the latter is more abundant, but unstable | Mytilus galloprovincialis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
temperature profile | Mytilus galloprovincialis |
| 3.2.1.1 | 35 | - |
enzyme form II | Mytilus galloprovincialis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
pH profile | Mytilus galloprovincialis |
| 3.2.1.1 | 6.5 | - |
enzyme form II | Mytilus galloprovincialis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
