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Literature summary extracted from

  • Saneyoshi, T.; Kume, S.; Mikoshiba, K.
    Calcium/calmodulin-dependent protein kinase I in Xenopus laevis (2003), Comp. Biochem. Physiol. B, 134B, 499-507.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.17 Calmodulin dependent on Xenopus laevis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.17 isozyme CaM-KI LiKbeta, DNA and amino acid sequence determination and analysis, overexpression of a constitutively active CaM-KI LiKbeta mutant inhibits cell division in frog embryos, and causes severe changes in HeLa cell morphology Xenopus laevis
2.7.11.17 isozyme CaM-KIalpha, DNA and amino acid sequence determination and analysis, overexpression of a constitutively active CaM-KIalpha mutant inhibits cell division in frog embryos, and causes severe changes in HeLa cell morphology Xenopus laevis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.17 K50E site-directed mutagenesis and truncation mutation constructing mutant K50E 1-295, the mutant isozyme CaM-KIalpha is constitutively active Xenopus laevis
2.7.11.17 K53E site-directed mutagenesis and truncation mutation constructing mutant K50E 1-297, the mutant isozyme CaM-KI LiKbeta is constitutively active Xenopus laevis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.17 Ca2+ dependent on Xenopus laevis
2.7.11.17 Mg2+
-
 Xenopus laevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.17 43000
-
 x * 43000, amino acid sequence calculation, x * 45000, SDS-PAGE Xenopus laevis
2.7.11.17 43800
-
 x * 43800, amino acid sequence calculation, x * 45000, SDS-PAGE Xenopus laevis
2.7.11.17 45000
-
 x * 43000, amino acid sequence calculation, x * 45000, SDS-PAGE Xenopus laevis
2.7.11.17 45000
-
 x * 43800, amino acid sequence calculation, x * 45000, SDS-PAGE Xenopus laevis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.17 additional information Xenopus laevis CaM-KI plays a role in cell structure regulation during early embryonic development ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.17 Xenopus laevis Q8AYR3 CaM-KI; two isozymes CaM-KI or CaM-KI LiKbeta, and CaM-KIalpha
-
2.7.11.17 Xenopus laevis Q8AYR3 CaM-KIalpha; two isozymes CaM-KI or CaM-KI LiKbeta, and CaM-KIalpha
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.17 oocyte high expression level of isozyme CaM-KI LiKbeta in the neurula stage Xenopus laevis
-
2.7.11.17 oocyte the isozymes CaM-KIalpha is constitutively expressed through embryogenesis leading Xenopus laevis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.17 ATP + syntide-2 i.e. PLARTLSVAGLPGKK Xenopus laevis ADP + phosphorylated syntide-2
-
 ?
2.7.11.17 additional information CaM-KI plays a role in cell structure regulation during early embryonic development Xenopus laevis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.17 ? x * 43000, amino acid sequence calculation, x * 45000, SDS-PAGE Xenopus laevis
2.7.11.17 ? x * 43800, amino acid sequence calculation, x * 45000, SDS-PAGE Xenopus laevis

Synonyms

EC Number Synonyms Comment Organism
2.7.11.17 calcium/calmodulin-dependent protein kinase I
-
 Xenopus laevis
2.7.11.17 calcium/calmodulin-dependent protein kinase Ialpha
-
 Xenopus laevis
2.7.11.17 CaM-KI
-
 Xenopus laevis
2.7.11.17 CaM-KI LiKbeta
-
 Xenopus laevis
2.7.11.17 CaM-KIalpha
-
 Xenopus laevis
2.7.11.17 XCaM-KI LiKbeta
-
 Xenopus laevis
2.7.11.17 XCaM-KIalpha
-
 Xenopus laevis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.17 30
-
 assay at Xenopus laevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.17 7.5
-
 assay at Xenopus laevis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.17 ATP
-
 Xenopus laevis