EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.6.1.24 | E46N | mutant shows an improved ApC/GpC preference with a 1450fold increase in comparison to wild-type activity | Aspergillus oryzae |
4.6.1.24 | Y45W/E46N | mutant shows an improved ApC/GpC preference with a 2100fold increase in comparison to wild-type activity | Aspergillus oryzae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.6.1.24 | additional information | - |
additional information | kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, 195 for mutant enzyme E46N and 137 for mutant enzyme E46N/Y45W | Aspergillus oryzae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.6.1.24 | Aspergillus oryzae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.6.1.24 | RNase T1 variants E46N and Y45W/E46N | Aspergillus oryzae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.6.1.24 | adenylyl-3',5'-cytidine + H2O | kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, 195 for mutant enzyme E46N and 137 for mutant enzyme E46N/Y45W | Aspergillus oryzae | adenosine 3'-phosphate + cytidine | - |
? | |
4.6.1.24 | guanylyl-3',5'-cytidine + H2O | kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, 195 for mutant enzyme E46N and 137 for mutant enzyme E46N/Y45W | Aspergillus oryzae | ? | - |
? | |
4.6.1.24 | RNA + H2O | - |
Aspergillus oryzae | Hydrolyzed RNA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.6.1.24 | RNase T1 | - |
Aspergillus oryzae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.6.1.24 | additional information | - |
additional information | kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, 195 for mutant enzyme E46N and 137 for mutant enzyme E46N/Y45W | Aspergillus oryzae |