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Literature summary extracted from

  • Buratai, L.B.; Nok, A.J.; Ibrahim, S.; Umar, I.A.; Esievo, K.A.
    Characterization of sialidase from bloodstream forms of Trypanosoma vivax (2006), Cell Biochem. Funct., 24, 71-77.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.18 dithiothreitol activates Trypanosoma vivax
3.2.1.18 dithiothreitol 1 mM, activates 2fold Trypanosoma vivax

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.18 (6R)-5-amino-2,6-anhydro-3,5-dideoxy-6-[(1R,2R)-1,2,3-trihydroxypropyl]-L-threo-hex-2-enonic acid competitive Trypanosoma vivax
3.2.1.18 2-deoxy-2,3 didehydro-N-acetyl-neuraminic acid
-
 Trypanosoma vivax
3.2.1.18 N-bromosuccinimide
-
 Trypanosoma vivax
3.2.1.18 p-hydroxy oxamic acid
-
 Trypanosoma vivax
3.2.1.18 p-hydroxymercuribenzoate 0.001 mM, complete inhibition Trypanosoma vivax
3.2.1.18 p-hydroxyoxamic acid competitive Trypanosoma vivax

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.18 0.17
-
 Neu5Ac(alpha2,3)-lactose pH 6.5, 37°C Trypanosoma vivax
3.2.1.18 0.17
-
 Neu5Ac(2-3)-alpha-lactose 37°C, pH 6.7 Trypanosoma vivax
3.2.1.18 0.45
-
 fetuin pH 6.5, 37°C Trypanosoma vivax
3.2.1.18 0.45
-
 fetuin 37°C, pH 6.7 Trypanosoma vivax

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.18 Cu2+ complete inhibition at 1 mM Trypanosoma vivax
3.2.1.18 Hg2+ complete inhibition at 1 mM Trypanosoma vivax

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.18 66000
-
 gel filtration Trypanosoma vivax
3.2.1.18 68000
-
 gel filtration Trypanosoma vivax

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.18 Trypanosoma vivax
-
 Agai strain
-
3.2.1.18 Trypanosoma vivax
-
 Agari strain
-
3.2.1.18 Trypanosoma vivax Agai
-
 Agai strain
-
3.2.1.18 Trypanosoma vivax Agari
-
 Agari strain
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.18 glycoprotein
-
 Trypanosoma vivax

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.18
-
 Trypanosoma vivax

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.18 bloodstream form
-
 Trypanosoma vivax
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.18 31
-
-
 Trypanosoma vivax
3.2.1.18 31.02
-
-
 Trypanosoma vivax

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.18 fetuin + H2O
-
 Trypanosoma vivax ?
-
 ?
3.2.1.18 fetuin + H2O
-
 Trypanosoma vivax Agai ?
-
 ?
3.2.1.18 fetuin + H2O
-
 Trypanosoma vivax Agari ?
-
 ?
3.2.1.18 fetuin + H2O
-
 Trypanosoma vivax Neu5Ac + ?
-
 ?
3.2.1.18 fetuin + H2O
-
 Trypanosoma vivax Agai Neu5Ac + ?
-
 ?
3.2.1.18 fetuin + H2O
-
 Trypanosoma vivax Agari Neu5Ac + ?
-
 ?
3.2.1.18 additional information the enzyme is inactive towards Neu5,9Ac2(alpha2,6)-lactose, colomic acid and the gangliosides GM1, and GDI Trypanosoma vivax ?
-
 ?
3.2.1.18 additional information the enzyme is inactive towards Neu5,9Ac2(alpha2,6)-lactose, colomic acid and the gangliosides GM1, and GDI Trypanosoma vivax Agai ?
-
 ?
3.2.1.18 additional information the enzyme is inactive towards Neu5,9Ac2(alpha2,6)-lactose, colomic acid and the gangliosides GM1, and GDI Trypanosoma vivax Agari ?
-
 ?
3.2.1.18 Neu5Ac(2-3)-alpha-lactose + H2O
-
 Trypanosoma vivax Neu5Ac + lactose
-
 ?
3.2.1.18 Neu5Ac(2-3)-alpha-lactose + H2O
-
 Trypanosoma vivax Agai Neu5Ac + lactose
-
 ?
3.2.1.18 Neu5Ac(2-3)-alpha-lactose + H2O
-
 Trypanosoma vivax Agari Neu5Ac + lactose
-
 ?
3.2.1.18 Neu5Ac(2-6)-alpha-lactose + H2O
-
 Trypanosoma vivax Neu5Ac + lactose
-
 ?
3.2.1.18 Neu5Ac(2-6)-alpha-lactose + H2O
-
 Trypanosoma vivax Agai Neu5Ac + lactose
-
 ?
3.2.1.18 Neu5Ac(2-6)-alpha-lactose + H2O
-
 Trypanosoma vivax Agari Neu5Ac + lactose
-
 ?
3.2.1.18 Neu5Ac(alpha2,3)lactose + H2O
-
 Trypanosoma vivax Neu5Ac + lactose
-
 ?
3.2.1.18 Neu5Ac(alpha2,6)-lactose + H2O
-
 Trypanosoma vivax ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.18 monomer 1 * 66000, SDS-PAGE Trypanosoma vivax

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.18 37
-
-
 Trypanosoma vivax

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.18 6.5
-
-
 Trypanosoma vivax

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.18 4 8.5 complete cessation of activity at pH 4 and 8.5 Trypanosoma vivax

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.18 0.06
-
 2-deoxy-2,3 didehydro-N-acetyl-neuraminic acid
-
 Trypanosoma vivax
3.2.1.18 0.06
-
 (6R)-5-amino-2,6-anhydro-3,5-dideoxy-6-[(1R,2R)-1,2,3-trihydroxypropyl]-L-threo-hex-2-enonic acid pH 6.5, 37°C Trypanosoma vivax
3.2.1.18 0.15
-
 p-hydroxy oxamic acid
-
 Trypanosoma vivax
3.2.1.18 0.15
-
 p-hydroxyoxamic acid pH 6.5, 37°C Trypanosoma vivax