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Literature summary extracted from

  • Arora, S.; Yang, J.M.; Kinzy, T.G.; Utsumi, R.; Okamoto, T.; Kitayama, T.; Ortiz, P.A.; Hait, W.N.
    Identification and characterization of an inhibitor of eukaryotic elongation factor 2 kinase against human cancer cell lines (2003), Cancer Res., 63, 6894-6899.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.20 Calmodulin
-
 Homo sapiens

Application

EC Number Application Comment Organism
2.7.11.20 pharmacology the enzyme is a target for development of anticancer drugs Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.20 overexpression of the enzyme in glioma T98-G cells causes a 10fold increased resistance to inhibitor NH125 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.20 additional information non-specific inhibitors of the enzyme cause cell death Homo sapiens
2.7.11.20 NH125 an imidazolium histidine kinase inhibitor also inhibits the eukaryotic eEF-2 kinase enzyme in vitro and in vivo, IC50 is 60 nM, decreases viability of cancer cell lines with IC50S of 0.0007 to 0.0048 mM, overview Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.20 Ca2+
-
 Homo sapiens
2.7.11.20 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.20 ATP + [elongation factor 2] Homo sapiens
-
 ADP + [elongation factor 2]phosphate
-
 ?
2.7.11.20 additional information Homo sapiens the enzyme expression and activity is increased in several forms of malignancy and human cancer, non-specific inhibitors of the enzyme cause cell death ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.20 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.20 A-172 cell glioblastoma cell line Homo sapiens
-
2.7.11.20 A-2780 cell ovarian carcinoma cell line Homo sapiens
-
2.7.11.20 HeLa cell cervical carcinoma cell line Homo sapiens
-
2.7.11.20 MCF-7 cell breast carcinoma cell line Homo sapiens
-
2.7.11.20 additional information the enzyme expression and activity is increased in several forms of malignancy Homo sapiens
-
2.7.11.20 OVCAR-3 cell ovarian carcinoma cell line Homo sapiens
-
2.7.11.20 PC-3 cell prostate carcinoma cell line Homo sapiens
-
2.7.11.20 T-98G cell glioblastoma cell line Homo sapiens
-
2.7.11.20 U-138MG cell glioblastoma cell line Homo sapiens
-
2.7.11.20 U-87MG cell glioblastoma cell line Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.20 ATP + [elongation factor 2]
-
 Homo sapiens ADP + [elongation factor 2]phosphate
-
 ?
2.7.11.20 additional information the enzyme expression and activity is increased in several forms of malignancy and human cancer, non-specific inhibitors of the enzyme cause cell death Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.20 eEF-2 kinase
-
 Homo sapiens
2.7.11.20 eukaryotic elongation factor 2 kinase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.20 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.20 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.20 ATP
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.11.20 0.00006
-
 an imidazolium histidine kinase inhibitor also inhibits the eukaryotic eEF-2 kinase enzyme in vitro and in vivo, IC50 is 60 nM Homo sapiens NH125
2.7.11.20 0.0007 0.0048 decreases viability of cancer cell lines with IC50S of 0.0007 to 0.0048 mM, overview Homo sapiens NH125