EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.23 | additional information | no inhibition by URB597, bovine serum albumin decreases the inhibitory effect of arachidonoyl-based compounds, e.g. VDM11 | Rattus norvegicus | |
3.1.1.23 | N-(4-hydroxy-2-methylphenyl)arachidonylamide | i.e. VDM11, an anandamide uptake inhibitor, substrate of fatty acid amide hydrolase, EC 3.5.1.4, IC50 is 0.021 mM | Rattus norvegicus | |
3.1.1.23 | N-(4-hydroxyphenyl)arachidonylamide | i.e. AM404, an anandamide uptake inhibitor, substrate of fatty acid amide hydrolase, EC 3.5.1.4, IC50 is 0.020 mM | Rattus norvegicus | |
3.5.1.4 | (5Z,8Z,11Z,14Z)-eicosantetraenoic acid 3-thienyl methyl ester | i.e. CAY-10402 | Rattus norvegicus | |
3.5.1.4 | 3'-carbamoyl-biphenyl-3-yl-cyclohexylcarbamate | i.e. URB597, specific | Rattus norvegicus | |
3.5.1.4 | N-(4-hydroxy-2-methylphenyl)arachidonylamide | i.e. VDM11, an anandamide uptake inhibitor, IC50 is 0.0026 mM, inhibitory potency depends upon the concentration of bovine serum albumin | Rattus norvegicus | |
3.5.1.4 | N-(4-hydroxyphenyl)arachidonylamide | i.e. AM404, an anandamide uptake inhibitor, IC50 is 0.0021 mM | Rattus norvegicus | |
3.5.1.99 | 1-oxazolo[4,5-b]pyridin-2-yl-9-octadecyn-1-one | - |
Rattus norvegicus | |
3.5.1.99 | 3'-carbamoyl-biphenyl-3-yl-cyclohexylcarbamate | - |
Rattus norvegicus | |
3.5.1.99 | N-(4-hydroxy-2-methylphenyl) arachidonoyl amide | i.e. VDM11, inhibits the metabolism of anandamide by rat brain FAAH. Inhibition may at least in part be a consequence of the compound acting as an alternative substrate | Rattus norvegicus | |
3.5.1.99 | N-(4-hydroxyphenyl)arachidonylamide | i.e. AM404, inhibits the metabolism of anandamide by rat brain FAAH | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.23 | cytosol | - |
Rattus norvegicus | 5829 | - |
3.1.1.23 | membrane | - |
Rattus norvegicus | 16020 | - |
3.5.1.4 | membrane | - |
Rattus norvegicus | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.23 | 2-arachidonoylglycerol + H2O | Rattus norvegicus | - |
arachidonic acid + glycerol | - |
? | |
3.1.1.23 | 2-arachidonoylglycerol + H2O | Rattus norvegicus Sprague-Dawley | - |
arachidonic acid + glycerol | - |
? | |
3.1.1.23 | 2-oleoylglycerol + H2O | Rattus norvegicus | - |
oleic acid + glycerol | - |
? | |
3.1.1.23 | 2-oleoylglycerol + H2O | Rattus norvegicus Sprague-Dawley | - |
oleic acid + glycerol | - |
? | |
3.5.1.4 | anandamide + H2O | Rattus norvegicus | i.e. arachidonoylethanolamide | ? | - |
? | |
3.5.1.4 | anandamide + H2O | Rattus norvegicus Sprague-Dawley | i.e. arachidonoylethanolamide | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.23 | Rattus norvegicus | - |
Sprague-Dawley rats | - |
3.5.1.4 | Rattus norvegicus | - |
Sprague-Dawley rats | - |
3.5.1.99 | Rattus norvegicus | - |
SpragueDawley | - |
3.5.1.99 | Rattus norvegicus Sprague-Dawley | - |
SpragueDawley | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.23 | brain | - |
Rattus norvegicus | - |
3.1.1.23 | cerebellum | - |
Rattus norvegicus | - |
3.5.1.4 | brain | - |
Rattus norvegicus | - |
3.5.1.4 | cerebellum | - |
Rattus norvegicus | - |
3.5.1.99 | brain | - |
Rattus norvegicus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.23 | 2-arachidonoylglycerol + H2O | - |
Rattus norvegicus | arachidonic acid + glycerol | - |
? | |
3.1.1.23 | 2-arachidonoylglycerol + H2O | - |
Rattus norvegicus Sprague-Dawley | arachidonic acid + glycerol | - |
? | |
3.1.1.23 | 2-oleoylglycerol + H2O | - |
Rattus norvegicus | oleic acid + glycerol | - |
? | |
3.1.1.23 | 2-oleoylglycerol + H2O | - |
Rattus norvegicus Sprague-Dawley | oleic acid + glycerol | - |
? | |
3.5.1.4 | anandamide + H2O | i.e. arachidonoylethanolamide | Rattus norvegicus | ? | - |
? | |
3.5.1.4 | anandamide + H2O | i.e. arachidonoylethanolamide or AEA | Rattus norvegicus | ? | - |
? | |
3.5.1.4 | anandamide + H2O | i.e. arachidonoylethanolamide | Rattus norvegicus Sprague-Dawley | ? | - |
? | |
3.5.1.4 | anandamide + H2O | i.e. arachidonoylethanolamide or AEA | Rattus norvegicus Sprague-Dawley | ? | - |
? | |
3.5.1.4 | N-(4-hydroxy-2-methylphenyl)arachidonylamide + H2O | i.e. VDM11, 15-20% of the activity with anandamide | Rattus norvegicus | 4-amino-3-cresol + arachidonic acid | - |
? | |
3.5.1.4 | N-(4-hydroxy-2-methylphenyl)arachidonylamide + H2O | i.e. VDM11, 15-20% of the activity with anandamide | Rattus norvegicus Sprague-Dawley | 4-amino-3-cresol + arachidonic acid | - |
? | |
3.5.1.4 | N-(4-hydroxyphenyl)arachidonylamide + H2O | i.e. AM404 | Rattus norvegicus | 4-aminophenol + arachidonic acid | - |
? | |
3.5.1.99 | anandamide + H2O | - |
Rattus norvegicus | ethanolamine + arachidonic acid | - |
? | |
3.5.1.99 | N-(4-hydroxy-2-methylphenyl) arachidonoyl amide + H2O | the rate of metabolism of VDM11 is about 1520% of that for anandamide | Rattus norvegicus | 4-amino-m-cresol + NH3 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.23 | MAGL | - |
Rattus norvegicus |
3.1.1.23 | monoacylglycerol lipase | - |
Rattus norvegicus |
3.5.1.4 | FAAH | - |
Rattus norvegicus |
3.5.1.4 | fatty acid amide hydrolase | - |
Rattus norvegicus |
3.5.1.99 | FAAH | - |
Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.23 | 37 | - |
assay at | Rattus norvegicus |
3.5.1.4 | 37 | - |
assay at | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.23 | 7.2 | - |
assay at | Rattus norvegicus |
3.5.1.4 | 7.2 | - |
assay at | Rattus norvegicus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.99 | 0.0021 | - |
N-(4-hydroxyphenyl)arachidonylamide | in the presence of fatty acid-free bovine serum albumin (0.125% w/v). In the absence of fatty acid-free bovine serum albumin, the IC50 value is reduced | Rattus norvegicus | |
3.5.1.99 | 0.0026 | - |
N-(4-hydroxy-2-methylphenyl) arachidonoyl amide | in the presence of fatty acid-free bovine serum albumin (0.125% w/v). In the absence of fatty acid-free bovine serum albumin, the IC50 value is reduced | Rattus norvegicus |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.1.1.23 | 0.02 | - |
i.e. AM404, an anandamide uptake inhibitor, substrate of fatty acid amide hydrolase, EC 3.5.1.4, IC50 is 0.020 mM | Rattus norvegicus | N-(4-hydroxyphenyl)arachidonylamide | |
3.1.1.23 | 0.021 | - |
i.e. VDM11, an anandamide uptake inhibitor, substrate of fatty acid amide hydrolase, EC 3.5.1.4, IC50 is 0.021 mM | Rattus norvegicus | N-(4-hydroxy-2-methylphenyl)arachidonylamide | |
3.5.1.4 | 0.0021 | - |
i.e. AM404, an anandamide uptake inhibitor, IC50 is 0.0021 mM | Rattus norvegicus | N-(4-hydroxyphenyl)arachidonylamide | |
3.5.1.4 | 0.0026 | - |
i.e. VDM11, an anandamide uptake inhibitor, IC50 is 0.0026 mM, inhibitory potency depends upon the concentration of bovine serum albumin | Rattus norvegicus | N-(4-hydroxy-2-methylphenyl)arachidonylamide |