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Literature summary extracted from

  • Yamazaki, T.; Akada, T.; Niizeki, O.; Suzuki, T.; Miyashita, H.; Sato, Y.
    Puromycin-insensitive leucyl-specific aminopeptidase (PILSAP) binds and catalyzes PDK1, allowing VEGF-stimulated activation of S6K for endothelial cell proliferation and angiogenesis (2004), Blood, 104, 2345-2352.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.1 expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), transient expression in COS-7 cells and in HEK-293 cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.1 E343A site-directed mutagenesis, inactive, dominant-negative mutant Mus musculus
3.4.11.1 E365A site-directed mutagenesis, inactive mutant Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 additional information vascular endothelial growth factor-stimulated G1/S transition in endothelial cells inhibits the enzyme activity and/or expression leading to an arrest in G1 phase Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Zn2+ metallopeptidase, contains the conserved motif HEXXH(18X)E Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.1 additional information Mus musculus the enzyme is required for DNA synthesis and plays an important role in angiogenesis by regulating the proliferation and migration of endothelial cells, mechanism via vascular endothelial growth factor and stimulation of S6 kinase, overview ?
-
 ?
3.4.11.1 phosphatidylinositol-dependent kinase-1 + H2O Mus musculus i.e. PDK1, removal of 9 amino acids from the N-terminus of the kinase, which allows S6 kinase to associate with PDK1 and the enzyme upon vascular endothelial growth factor stimulation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 recombinant enzyme partially from COS-7 cells Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 MSS31 cell
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-leucyl-7-amido-4-methylcoumarin + H2O
-
 Mus musculus L-leucine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 additional information the enzyme is required for DNA synthesis and plays an important role in angiogenesis by regulating the proliferation and migration of endothelial cells, mechanism via vascular endothelial growth factor and stimulation of S6 kinase, overview Mus musculus ?
-
 ?
3.4.11.1 phosphatidylinositol-dependent kinase-1 + H2O i.e. PDK1, removal of 9 amino acids from the N-terminus of the kinase, which allows S6 kinase to associate with PDK1 and the enzyme upon vascular endothelial growth factor stimulation Mus musculus ?
-
 ?
3.4.11.1 phosphatidylinositol-dependent kinase-1 + H2O i.e. PDK1, removal of 9 amino acids from the N-terminus of the kinase Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 PILSAP
-
 Mus musculus
3.4.11.1 puromycin-insensitive leucyl-specific aminopeptidase
-
 Mus musculus