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Literature summary extracted from

  • Gupta, P.; Saleemuddin, M.
    Bioaffinity based oriented immobilization of stem bromelain (2006), Biotechnol. Lett., 28, 917-922.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.22.32 enzyme affinity-bound to a Sepharose matrix precoupled with the lactin concanavalin A is more stable to thermal inactivation than native enzyme or enzyme covalently coupled to the CNBr-activated Sepharose Ananas comosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.32 additional information
-
 additional information Km-for casein is 1.1 mg/ml for the native enzyme, 2 mg/ml for the enzyme covalently coupled to the CNBr-activated Sepharose and 0.54 mg/ml for enzyme affinity-bound to a Sepharose matrix precoupled with the lactin concanavalin A Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.32 Ananas comosus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.32 glycoprotein contains a single oligosaccharide chain attached to the polypeptide Ananas comosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.32 stem
-
 Ananas comosus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.32 casein + H2O
-
 Ananas comosus hydrolyzed casein
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.32 60
-
 100 min, native enzyme loses 80% of initial activity, enzyme covalently coupled to the CNBr-activated Sepharose loses 70% of the initial activity, enzyme affinity-bound to a Sepharose matrix precoupled with the lactin concanavalin A loses 50% of initial activity Ananas comosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.32 7.5
-
 native enzyme, enzyme covalently coupled to the CNBr-activated Sepharose and enzyme affinity-bound to a Sepharose matrix precoupled with the lactin concanavalin A Ananas comosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.32 5 11 pH 5.0: native enzyme shows about 25% of maximal activity, enzyme covalently coupled to the CNBr-activated Sepharose shows about 30% of maximal activity, enzyme affinity-bound to a Sepharose matrix precoupled with the lactin concanavalin A shows about 35% of maximal activity, pH 11: native enzyme shows about 25% of maximal activity, enzyme covalently coupled to the CNBr-activated Sepharose shows about 30% of maximal activity, enzyme affinity-bound to a Sepharose matrix precoupled with the lactin concanavalin A shows about 60% of maximal activity Ananas comosus