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Literature summary extracted from

  • Li, X.; Pei, J.; Wu, G.; Shao, W.
    Expression, purification and characterization of a recombinant beta-glucosidase from Volvariella volvacea (2005), Biotechnol. Lett., 27, 1369-1373.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.21 additional information the enzyme is unaffected by EDTA and Tween 20 Volvariella volvacea
3.2.1.21 Triton X-100
-
 Volvariella volvacea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 overexpression in Escherichia coli Volvariella volvacea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Cu2+ complete inhibition Volvariella volvacea
3.2.1.21 Hg2+ complete inhibition Volvariella volvacea
3.2.1.21 additional information the enzyme is unaffected by EDTA and Tween 20 Volvariella volvacea
3.2.1.21 Ni2+
-
 Volvariella volvacea
3.2.1.21 SDS complete inhibition Volvariella volvacea
3.2.1.21 Zn2+
-
 Volvariella volvacea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.2
-
 4-nitrophenyl beta-D-glucoside pH 6.4, 50°C, recombinant enzyme Volvariella volvacea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 additional information the enzyme is unaffected by Mn2+, Mg2+, and Co2+ Volvariella volvacea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 97000
-
 4 * 97000, recombinant enzyme, SDS-PAGE Volvariella volvacea
3.2.1.21 380000
-
 recombinant enzyme, gel filtration Volvariella volvacea

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Volvariella volvacea Q9C3Z9
-
-
3.2.1.21 Volvariella volvacea V1-1 Q9C3Z9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 recombinant enzyme 16fold to homogeneity by ion exchange chromatography and gel filtration Volvariella volvacea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 96
-
 purified recombinant enzyme Volvariella volvacea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl alpha-D-xylopyranoside + H2O
-
 Volvariella volvacea 4-nitrophenol + D-xylose
-
 ?
3.2.1.21 4-nitrophenyl alpha-D-xylopyranoside + H2O
-
 Volvariella volvacea V1-1 4-nitrophenol + D-xylose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-cellobioside + H2O
-
 Volvariella volvacea ?
-
 ?
3.2.1.21 4-nitrophenyl beta-D-cellobioside + H2O
-
 Volvariella volvacea V1-1 ?
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Volvariella volvacea 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Volvariella volvacea V1-1 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Volvariella volvacea beta-D-glucose + beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Volvariella volvacea V1-1 beta-D-glucose + beta-D-glucose
-
 ?
3.2.1.21 additional information no activity with oat spelt xylan, 4-nitrophenyl-alpha-D-arabinofuranoside, and 4-nitrophenyl-beta-D-xylanopyranoside Volvariella volvacea ?
-
 ?
3.2.1.21 additional information no activity with oat spelt xylan, 4-nitrophenyl-alpha-D-arabinofuranoside, and 4-nitrophenyl-beta-D-xylanopyranoside Volvariella volvacea V1-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 tetramer 4 * 97000, recombinant enzyme, SDS-PAGE Volvariella volvacea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 50
-
-
 Volvariella volvacea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 45
-
 purified recombinant enzyme, half-life 1 h Volvariella volvacea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6.4
-
-
 Volvariella volvacea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 5.6 8 stable, purified recombinant enzyme Volvariella volvacea