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Literature summary extracted from

  • Kawase, T.; Yokokawa, S.; Saito, A.; Fuji, T.; Nikaidou, N.; Miyashita, K.; Watanabe, T.
    Comparison of enzymatic and antifungal properties between family 18 and 19 chitinases from S. coelicolor A3(2) (2006), Biosci. Biotechnol. Biochem., 70, 988-998.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 13 genes, DNA and amino asequence determination and analysis, expression in Escherichia coli strains JM109 and BL21(DE3), sequence and domain structure comparison, overview Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 chitin + H2O Streptomyces coelicolor
-
?
-
?
3.2.1.14 chitin + H2O Streptomyces coelicolor A3(2)
-
?
-
?
3.2.1.14 additional information Streptomyces coelicolor the enzyme plays a role in pathogen defense ?
-
?
3.2.1.14 additional information Streptomyces coelicolor A3(2) the enzyme plays a role in pathogen defense ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Streptomyces coelicolor
-
13 genes encoding 11 enzyme from chitinase family 18 and 19, overview
-
3.2.1.14 Streptomyces coelicolor A3(2)
-
13 genes encoding 11 enzyme from chitinase family 18 and 19, overview
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 recombinant isozymes Chi18bA, Chi19F, Chi18aC and Chi18aD from Escherichia coli strain BL21(DE3) by hydroxyapatite chromatography and dialysis, recombinant isozymes Chi18bA, Chi19F from Escherichia coli strain BL21(DE3) by chitin affinity chromatography and dialysis, all enzyme are lyophilized Streptomyces coelicolor

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 additional information
-
activity of recombinant isozymes Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O
-
Streptomyces coelicolor ?
-
?
3.2.1.14 chitin + H2O coloidal chitin and glycol chitin prepared from commercial powdered chitin, prawn shell chitin, crystalline beta-chitin, soluble chitin Streptomyces coelicolor ?
-
?
3.2.1.14 chitin + H2O
-
Streptomyces coelicolor A3(2) ?
-
?
3.2.1.14 chitin + H2O coloidal chitin and glycol chitin prepared from commercial powdered chitin, prawn shell chitin, crystalline beta-chitin, soluble chitin Streptomyces coelicolor A3(2) ?
-
?
3.2.1.14 chitosan 7B + H2O 70% deacetylated Streptomyces coelicolor ?
-
?
3.2.1.14 chitosan 7B + H2O 70% deacetylated Streptomyces coelicolor A3(2) ?
-
?
3.2.1.14 additional information the enzyme plays a role in pathogen defense Streptomyces coelicolor ?
-
?
3.2.1.14 additional information chitin substrate specificities of recombinant isozymes, overview Streptomyces coelicolor ?
-
?
3.2.1.14 additional information the enzyme plays a role in pathogen defense Streptomyces coelicolor A3(2) ?
-
?
3.2.1.14 additional information chitin substrate specificities of recombinant isozymes, overview Streptomyces coelicolor A3(2) ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 More the enzymes belong to the chitinase families 18, Chi18aC and Chi18aD, and family 19, isozymes Chi18bA and Chi19F Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 50
-
recombinant isozymes Chi18bA and Chi19F Streptomyces coelicolor
3.2.1.14 60
-
recombinant isozymes Chi18aC and Chi18aD Streptomyces coelicolor

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 30 70 temperature profile of recombinant isozymes, overview Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 additional information
-
the pH optimum depends strongly on the chitin substrate used Streptomyces coelicolor
3.2.1.14 2
-
recombinant isozyme Chi18aC Streptomyces coelicolor
3.2.1.14 3 4 recombinant isozyme Chi18aD Streptomyces coelicolor
3.2.1.14 4 8 recombinant isozyme Chi19F, dependent on substrate Streptomyces coelicolor
3.2.1.14 4 6 recombinant isozyme Chi18bA, dependent on substrate Streptomyces coelicolor