EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.2.2 | full-length enzyme and catalytic modules CM9-1 and CM9-2 are separately expressed in Escherichia coli | Thermoclostridium stercorarium |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.2 | Ca2+ | absolute requirement, maximal activity of recombinantly expressed full-length enzyme at 0.05 mM, maximal activity of recombinantly expressed catalytic module CM9-1 at 0.02 mM, maximal activity of recombinantly expressed catalytic module CM9-2 at 0.1 mM | Thermoclostridium stercorarium |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.2.2 | 58513 | - |
x * 58513, recombinantly expressed catalytic module CM9-1, SDS-PAGE | Thermoclostridium stercorarium |
4.2.2.2 | 76383 | - |
x * 76383, recombinantly expressed catalytic module CM9-2, SDS-PAGE | Thermoclostridium stercorarium |
4.2.2.2 | 133297 | - |
x * 133297, recombinant full-length enzyme, SDS-PAGE | Thermoclostridium stercorarium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.2.2 | Thermoclostridium stercorarium | Q76EC9 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.2.2 | pectin | apple pectin, citrus pectin, as the percentage of methylation in pectin becomes higher, the activities become lower | Thermoclostridium stercorarium | ? | - |
? | |
4.2.2.2 | polygalacturonic acid | - |
Thermoclostridium stercorarium | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.2.2 | ? | x * 133297, recombinant full-length enzyme, SDS-PAGE | Thermoclostridium stercorarium |
4.2.2.2 | ? | x * 58513, recombinantly expressed catalytic module CM9-1, SDS-PAGE | Thermoclostridium stercorarium |
4.2.2.2 | ? | x * 76383, recombinantly expressed catalytic module CM9-2, SDS-PAGE | Thermoclostridium stercorarium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.2.2 | Pel9A | - |
Thermoclostridium stercorarium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.2 | 65 | - |
- |
Thermoclostridium stercorarium |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.2 | 70 | - |
pH 7, 10 min, absence of substrate, stable | Thermoclostridium stercorarium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.2 | 7 | - |
- |
Thermoclostridium stercorarium |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.2 | 3 | 10 | 4°C, 12 h, stable | Thermoclostridium stercorarium |