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Literature summary extracted from

  • Kabir, K.E.; Sugimoto, H.; Tado, H.; Endo, K.; Yamanaka, A.; Tanaka, S.; Koga, D.
    Effect of Bombyx mori chitiniase against Japanese pine sawyer (Monochamus alternatus) adults as a biopesticide (2006), Biosci. Biotechnol. Biochem., 70, 219-229.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.14 agriculture the enzyme of Bomby mori can acts as a biopesticide in insects Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 75000
-
 x * 75000 Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 chitin + H2O Bombyx mori the enzyme plays a role in pathogen defense and serves as biocontrol agent against the adult Japanese pine sawyer, Monochamus alternatus, oral ingestion of the enzyme by the insect causes high mortality, the enzyme degrades peritrophic membrane chitin in the beetle, but does not affect the midgut epithelium ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Bombyx mori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 native enzyme by three chromatographical steps Bombyx mori

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 additional information
-
 enzyme dose-dependent bark consumption and mortality of Japanese pine sawyer beetles, overview Bombyx mori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O
-
 Bombyx mori ?
-
 ?
3.2.1.14 chitin + H2O the enzyme plays a role in pathogen defense and serves as biocontrol agent against the adult Japanese pine sawyer, Monochamus alternatus, oral ingestion of the enzyme by the insect causes high mortality, the enzyme degrades peritrophic membrane chitin in the beetle, but does not affect the midgut epithelium Bombyx mori ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 75000 Bombyx mori

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 CHI
-
 Bombyx mori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 25
-
 oral bioassay in Japanese pine sawyer beetles Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 8
-
 oral bioassay in Japanese pine sawyer beetles Bombyx mori