Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tamoi, M.; Nagaoka, M.; Shigeoka, S.
    Immunological properties of sedoheptulose-1,7-bisphosphatase from Chlamydomonas sp. W80 (2005), Biosci. Biotechnol. Biochem., 69, 848-851.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.37 functional overexpression as soluble His-tagged enzyme in Escherichia coli Chlamydomonas sp. W80

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.37 chloroplast stroma
-
 Arabidopsis thaliana 9570
-
3.1.3.37 chloroplast stroma
-
 Chlamydomonas sp. W80 9570
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.37 Mg2+ required Arabidopsis thaliana
3.1.3.37 Mg2+ required Chlamydomonas sp. W80

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.37 41600
-
 x * 41600, recombinant enzyme, SDS-PAGE Chlamydomonas sp. W80

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.37 sedoheptulose 1,7-bisphosphate + H2O Arabidopsis thaliana the enzyme is involved in the generation of CO2-acceptor ribulose 1,5-bisphosphate in the Calvin cycle sedoheptulose 7-phosphate + phosphate
-
 ir
3.1.3.37 sedoheptulose 1,7-bisphosphate + H2O Chlamydomonas sp. W80 the enzyme is involved in the generation of CO2-acceptor ribulose 1,5-bisphosphate in the Calvin cycle sedoheptulose 7-phosphate + phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.37 Arabidopsis thaliana
-
-
-
3.1.3.37 Chlamydomonas sp. W80
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.37 recombinant His-tagged enzyme from Escherichia coli in one step by nickel affinity chelating chromatography Chlamydomonas sp. W80

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.37 flower
-
 Arabidopsis thaliana
-
3.1.3.37 leaf
-
 Arabidopsis thaliana
-
3.1.3.37 additional information expression profile of the enzyme in plant tissues, no activity and expression in roots Arabidopsis thaliana
-
3.1.3.37 silique green Arabidopsis thaliana
-
3.1.3.37 stem
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.37 0.224
-
 leaf enzyme extract Arabidopsis thaliana
3.1.3.37 7.15
-
 recombinant enzyme in the soluble fraction of Escherichia coli Chlamydomonas sp. W80

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.37 sedoheptulose 1,7-bisphosphate + H2O
-
 Arabidopsis thaliana sedoheptulose 7-phosphate + phosphate
-
 ir
3.1.3.37 sedoheptulose 1,7-bisphosphate + H2O
-
 Chlamydomonas sp. W80 sedoheptulose 7-phosphate + phosphate
-
 ir
3.1.3.37 sedoheptulose 1,7-bisphosphate + H2O the enzyme is involved in the generation of CO2-acceptor ribulose 1,5-bisphosphate in the Calvin cycle Arabidopsis thaliana sedoheptulose 7-phosphate + phosphate
-
 ir
3.1.3.37 sedoheptulose 1,7-bisphosphate + H2O the enzyme is involved in the generation of CO2-acceptor ribulose 1,5-bisphosphate in the Calvin cycle Chlamydomonas sp. W80 sedoheptulose 7-phosphate + phosphate
-
 ir

Subunits

EC Number Subunits Comment Organism
3.1.3.37 ? x * 41600, recombinant enzyme, SDS-PAGE Chlamydomonas sp. W80

Synonyms

EC Number Synonyms Comment Organism
3.1.3.37 SBPase
-
 Arabidopsis thaliana
3.1.3.37 SBPase
-
 Chlamydomonas sp. W80
3.1.3.37 sedoheptulose-1,7-bisphosphatase
-
 Arabidopsis thaliana
3.1.3.37 sedoheptulose-1,7-bisphosphatase
-
 Chlamydomonas sp. W80

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.37 8
-
 assay at Arabidopsis thaliana
3.1.3.37 8
-
 assay at Chlamydomonas sp. W80