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Literature summary extracted from

  • Si Si, H.; Kurokawa, J.; Suryani; Kimura, T.; Ohmiya, K.; Sakka, K.
    A novel thermophilic pectate lyase containing two catalytic modules of Clostridium stercorarium (2005), Biosci. Biotechnol. Biochem., 69, 2138-2145.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.2
-
 Thermoclostridium stercorarium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ca2+ strong activation, optimal activity at 0.05 mM CaCl2 Thermoclostridium stercorarium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 135171
-
 x * 135171, calculated from sequence Thermoclostridium stercorarium

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Thermoclostridium stercorarium Q76EC9
-
-
4.2.2.2 Thermoclostridium stercorarium F-9 Q76EC9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2 recombinant Thermoclostridium stercorarium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 pectin apple or citrus pectin, activity with pectin is lower than activity with polygalacturonate Thermoclostridium stercorarium ?
-
 ?
4.2.2.2 pectin apple or citrus pectin, activity with pectin is lower than activity with polygalacturonate Thermoclostridium stercorarium F-9 ?
-
 ?
4.2.2.2 polygalacturonic acid
-
 Thermoclostridium stercorarium ?
-
 ?
4.2.2.2 polygalacturonic acid
-
 Thermoclostridium stercorarium F-9 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 ? x * 135171, calculated from sequence Thermoclostridium stercorarium

Synonyms

EC Number Synonyms Comment Organism
4.2.2.2 pectate lyase 9A
-
 Thermoclostridium stercorarium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 65
-
-
 Thermoclostridium stercorarium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.2 50 70 about 30% of maximal activity at 50°C and at 70°C Thermoclostridium stercorarium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.2 70
-
 10 min, stable up to Thermoclostridium stercorarium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 7
-
-
 Thermoclostridium stercorarium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.2 3 10 4°C, 12 h, stable in the range Thermoclostridium stercorarium