Literature summary extracted from
An, C.L.; Lim, W.J.; Hong, S.Y.; Kim, E.J.; Shin, E.C.; Kim, M.K.; Lee, J.R.; Park, S.R.; Woo, J.G.; Lim, Y.P.; Yun, H.D.
Analysis of bgl operon structure and characterization of beta-glucosidase from Pectobacterium carotovorum subsp. carotovorum LY34 (2004), Biosci. Biotechnol. Biochem., 68, 2270-2278.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.21 |
bgl operon, gene bglB, contruction of a genomic library, DNA and amino acid sequence determination and analysis, determination of bgl operon structure and organization, overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) as His-tagged proteins, subcloning in Escherichia coli strain DH5alpha |
Pectobacterium carotovorum |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.2.1.21 |
E173A |
site-directed mutagenesis, inactive mutant |
Pectobacterium carotovorum |
3.2.1.21 |
E362A |
site-directed mutagenesis, inactive mutant |
Pectobacterium carotovorum |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.2.1.21 |
Co2+ |
85% inhibition at 2 mM |
Pectobacterium carotovorum |
|
3.2.1.21 |
Cu2+ |
95% inhibition at 2 mM |
Pectobacterium carotovorum |
|
3.2.1.21 |
Hg2+ |
complete inhibition at 2 mM |
Pectobacterium carotovorum |
|
3.2.1.21 |
Mn2+ |
28% inhibition at 2 mM |
Pectobacterium carotovorum |
|
3.2.1.21 |
Zn2+ |
95% inhibition at 2 mM |
Pectobacterium carotovorum |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.2.1.21 |
0.25 |
- |
4-nitrophenyl beta-D-glucopyranoside |
pH 7.0, 40°C, recombinant enzyme |
Pectobacterium carotovorum |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.2.1.21 |
Mg2+ |
activates, maximal at 15 mM, 20% activation at 2 mM |
Pectobacterium carotovorum |
|
3.2.1.21 |
additional information |
no effect by Ca2+ at 2 mM |
Pectobacterium carotovorum |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.21 |
Pectobacterium carotovorum |
Q6QGY5 |
ssp. carotovorum, strain LY34, bgl operon |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.21 |
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
Pectobacterium carotovorum |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
3.2.1.21 |
celloheptaose + 6 H2O = 7 beta-D-glucose |
residues E173 and E362 are essential for catalytic activity |
Pectobacterium carotovorum |
|
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.2.1.21 |
553 |
- |
purified recombinant enzyme, substrate 4-nitrophenyl beta-D-glucopyranoside |
Pectobacterium carotovorum |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.2.1.21 |
4-nitrophenyl beta-D-glucopyranoside + H2O |
hydrolysis of beta-linkages |
Pectobacterium carotovorum |
4-nitrophenol + beta-D-glucose |
- |
? |
|
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.2.1.21 |
40 |
- |
- |
Pectobacterium carotovorum |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
3.2.1.21 |
20 |
50 |
25% of maximal activity at 20°C and 50°C, half-maximal activity at 28°C and 46°C |
Pectobacterium carotovorum |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.2.1.21 |
40 |
- |
loss of 40% of activity within 50 min |
Pectobacterium carotovorum |
3.2.1.21 |
50 |
- |
loss of 90% of activity within 20 min |
Pectobacterium carotovorum |
3.2.1.21 |
60 |
- |
inactivation within 20 min |
Pectobacterium carotovorum |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.2.1.21 |
7 |
- |
- |
Pectobacterium carotovorum |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.2.1.21 |
4 |
9 |
20% of maximal activity at pH 5.0 and pH 9.0, half-maximal activity at pH 5.5 and pH 8.5 |
Pectobacterium carotovorum |