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Literature summary extracted from

  • Xu, X.; Kashima, O.; Saito, A.; Azakami, H.; Kato, A.
    Structural and functional properties of chicken lysozyme fused serine-rich heptapeptides at the C-terminus (2004), Biosci. Biotechnol. Biochem., 68, 1273-1278.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.17 two serine-rich heptapeptides, Ser-Ser-Ser-Lys-Ser-Ser-Ser (S6K) and Ser-Ser-Ser-Ser-Ser-Ser-Ser (S7) are fused to the C-terminus of chicken lysozyme by genetic modification. The cDNAs of S6K-lysozyme and S7-lysozyme are inserted into the expression vector of Pichia pastoris and secreted in the yeast cultivation medium. The secretion amounts of S6K-lysozyme and S7-lysozyme are about 60% of that of wild-type lysozyme Gallus gallus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.17 additional information two serine-rich heptapeptides, Ser-Ser-Ser-Lys-Ser-Ser-Ser (S6K) and Ser-Ser-Ser-Ser-Ser-Ser-Ser (S7) are fused to the C-terminus of chicken lysozyme by genetic modification. The cDNAs of S6K-lysozyme and S7-lysozyme are inserted into the expression vector of Pichia pastoris and secreted in the yeast cultivation medium. The secretion amounts of S6K-lysozyme and S7-lysozyme are about 60% of that of wild-type lysozyme. The bactericidal activity against Escherichia coli of S6L-lysozyme and S7-lysozyme is greatly increased Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.17 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.17 two serine-rich heptapeptides, Ser-Ser-Ser-Lys-Ser-Ser-Ser (S6K) and Ser-Ser-Ser-Ser-Ser-Ser-Ser (S7) are fused to the C-terminus of chicken lysozyme by genetic modification. The cDNAs of S6K-lysozyme and S7-lysozyme are inserted into the expression vector of Pichia pastoris and secreted in the yeast cultivation medium Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.17 additional information two serine-rich heptapeptides, Ser-Ser-Ser-Lys-Ser-Ser-Ser (S6K) and Ser-Ser-Ser-Ser-Ser-Ser-Ser (S7) are fused to the C-terminus of chicken lysozyme by genetic modification. The cDNAs of S6K-lysozyme and S7-lysozyme are inserted into the expression vector of Pichia pastoris and secreted in the yeast cultivation medium. The secretion amounts of S6K-lysozyme and S7-lysozyme are about 60% of that of wild-type lysozyme Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.17 peptidoglycan + H2O lysis of Micrococcus lysodeikticus cells Gallus gallus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.17 76
-
 mid-transition temperature is 76.3°C for S6K-lysozyme and 76.0°C for S7-lysozyme Gallus gallus
3.2.1.17 77
-
 mid-transition temperature of wild-type enzyme Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.17 6
-
 lysis of Micrococcus lysodeikticus cells Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.17 5 7 pH 5.0: wild-type enzyme shows about 80% of maximal activity, S6K-lysozyme and S7-lysozyme shows about 60% of maximal activity, pH 7.0: about 40% of maximal activity, wild-type enzyme, S6K-lysozyme and S7-lysozyme, lysis of Micrococcus lysodeikticus cells Gallus gallus