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Literature summary extracted from

  • Suresh, C.; Kitaoka, M.; Hayashi, K.
    A thermostable non-xylanolytic alpha-glucuronidase of Thermotoga maritima MSB8 (2003), Biosci. Biotechnol. Biochem., 67, 2359-2364.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.139 2-mercaptoethanol maximal activation at 150 mM Thermotoga maritima
3.2.1.139 DTT activates Thermotoga maritima
3.2.1.139 L-cysteine maximal activation at 0.6 mM, 15% of the activation with 2-mercaptoethanol Thermotoga maritima
3.2.1.139 NAD+ maximal activation at 0.5 mM Thermotoga maritima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.139 expression in Escherichia coli Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.139 Mn2+ required Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.139 0.1
-
p-nitrophenyl alpha-D-glucuronopyranoside
-
Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.139 Thermotoga maritima P96105
-
-
3.2.1.139 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 P96105
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.139 recombinant Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.139 p-nitrophenyl alpha-D-glucuronopyranoside + H2O
-
Thermotoga maritima p-nitrophenol + D-glucuronate
-
?
3.2.1.139 p-nitrophenyl alpha-D-glucuronopyranoside + H2O
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 p-nitrophenol + D-glucuronate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.139 Agu4B
-
Thermotoga maritima
3.2.1.139 non-xylanolytic alpha-glucuronidase
-
Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.139 80
-
-
Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.139 60 90 60°C: about 50% of maximal activity, 90°C: about 80% of maximal activity Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.139 70
-
30 min, stable up to Thermotoga maritima
3.2.1.139 80
-
30 min, 10% loss of activity Thermotoga maritima
3.2.1.139 90
-
30 min, complete inactivation Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.139 0.28
-
p-nitrophenyl alpha-D-glucuronopyranoside
-
Thermotoga maritima

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.139 4 12 30 min, stable between pH 4 and pH 12 Thermotoga maritima