EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.139 | 2-mercaptoethanol | maximal activation at 150 mM | Thermotoga maritima | |
3.2.1.139 | DTT | activates | Thermotoga maritima | |
3.2.1.139 | L-cysteine | maximal activation at 0.6 mM, 15% of the activation with 2-mercaptoethanol | Thermotoga maritima | |
3.2.1.139 | NAD+ | maximal activation at 0.5 mM | Thermotoga maritima |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.139 | expression in Escherichia coli | Thermotoga maritima |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.139 | Mn2+ | required | Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.139 | 0.1 | - |
p-nitrophenyl alpha-D-glucuronopyranoside | - |
Thermotoga maritima |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.139 | Thermotoga maritima | P96105 | - |
- |
3.2.1.139 | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | P96105 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.139 | recombinant | Thermotoga maritima |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.139 | p-nitrophenyl alpha-D-glucuronopyranoside + H2O | - |
Thermotoga maritima | p-nitrophenol + D-glucuronate | - |
? | |
3.2.1.139 | p-nitrophenyl alpha-D-glucuronopyranoside + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | p-nitrophenol + D-glucuronate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.139 | Agu4B | - |
Thermotoga maritima |
3.2.1.139 | non-xylanolytic alpha-glucuronidase | - |
Thermotoga maritima |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.139 | 80 | - |
- |
Thermotoga maritima |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.139 | 60 | 90 | 60°C: about 50% of maximal activity, 90°C: about 80% of maximal activity | Thermotoga maritima |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.139 | 70 | - |
30 min, stable up to | Thermotoga maritima |
3.2.1.139 | 80 | - |
30 min, 10% loss of activity | Thermotoga maritima |
3.2.1.139 | 90 | - |
30 min, complete inactivation | Thermotoga maritima |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.139 | 0.28 | - |
p-nitrophenyl alpha-D-glucuronopyranoside | - |
Thermotoga maritima |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.139 | 4 | 12 | 30 min, stable between pH 4 and pH 12 | Thermotoga maritima |