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Literature summary extracted from

  • Nishiwaki, T.; Hayashi, K.
    Purification and characterization of an aminopeptidase from the edible basidiomycete Grifola frondosa (2001), Biosci. Biotechnol. Biochem., 65, 424-427.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.11.22 nutrition the enzyme is useful to reduce bitterness of food products by hydrolysis of peptides with hydrophobic amino acids causing the bitter taste Grifola frondosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.22 1,10-phenanthroline inactivation Grifola frondosa
3.4.11.22 bestatin strong inhibition Grifola frondosa
3.4.11.22 Co2+ 66% inhibition at 0.03 mM Grifola frondosa
3.4.11.22 Cu2+ 32% inhibition at 0.03 mM Grifola frondosa
3.4.11.22 EDTA inactivation Grifola frondosa
3.4.11.22 Mg2+ complete inhibition at 0.03 mM Grifola frondosa
3.4.11.22 Mn2+ 94% inhibition at 0.03 mM Grifola frondosa
3.4.11.22 additional information no inhibition by DFP Grifola frondosa
3.4.11.22 Zn2+ 42% inhibition at 0.03 mM Grifola frondosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.22 0.09
-
 L-Val-4-nitroanilide pH 8.5, 65°C Grifola frondosa
3.4.11.22 0.31
-
 L-Phe-4-nitroanilide pH 8.5, 65°C Grifola frondosa
3.4.11.22 0.35
-
 L-Leu-4-nitroanilide pH 8.5, 65°C Grifola frondosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.22 additional information the enzyme is no metallopeptidase Grifola frondosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.22 27000
-
 gel filtration Grifola frondosa
3.4.11.22 30000
-
 1 * 30000, SDS-PAGE Grifola frondosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.22 Grifola frondosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.22 native enzyme 178fold to homogeneity by ammonium sulfate fractionation, hydrophobic interaction chromatography, gel filtration, and ion exchange chromatography Grifola frondosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.22 8.55
-
 purified enzyme Grifola frondosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.22 L-Leu-4-nitroanilide + H2O best substrate Grifola frondosa L-Leu + 4-nitroaniline
-
 ?
3.4.11.22 L-Phe-4-nitroanilide + H2O
-
 Grifola frondosa L-Phe + 4-nitroaniline
-
 ?
3.4.11.22 L-Val-4-nitroanilide + H2O low activity Grifola frondosa L-Val + 4-nitroaniline
-
 ?
3.4.11.22 additional information the enzyme prefers peptide substrates with an N-terminal hydrophobic amino acid residue Grifola frondosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.22 monomer 1 * 30000, SDS-PAGE Grifola frondosa

Synonyms

EC Number Synonyms Comment Organism
3.4.11.22 Aminopeptidase
-
 Grifola frondosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.22 65
-
-
 Grifola frondosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.22 55
-
 60 min, purified enzyme, stable Grifola frondosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.22 0.02
-
 L-Val-4-nitroanilide pH 8.5, 65°C Grifola frondosa
3.4.11.22 0.95
-
 L-Phe-4-nitroanilide pH 8.5, 65°C Grifola frondosa
3.4.11.22 1.43
-
 L-Leu-4-nitroanilide pH 8.5, 65°C Grifola frondosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.22 8.5
-
-
 Grifola frondosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.22 6 10.5 60 min, purified enzyme, stable Grifola frondosa