Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yamauchi, Y.; Ejiri, Y.; Tanaka, K.
    Purification of an aminopeptidase preferentially releasing N-terminal alanine from cucumber leaves and its identification as a plant aminopeptidase N (2001), Biosci. Biotechnol. Biochem., 65, 2802-2805.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.2 1,10-phenanthroline complete inhibition Cucumis sativus
3.4.11.2 bestatin strong inhibition Cucumis sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.2 0.092
-
 L-Lys-2-naphthylamide pH 8.0, 37°C Cucumis sativus
3.4.11.2 0.099
-
 L-Arg-2-naphthylamide pH 8.0, 37°C Cucumis sativus
3.4.11.2 0.197
-
 L-Ala-2-naphthylamide pH 8.0, 37°C Cucumis sativus
3.4.11.2 0.31
-
 L-Met-4-nitroanilide pH 8.0, 37°C Cucumis sativus
3.4.11.2 0.45
-
 L-Ala-4-nitroanilide pH 8.0, 37°C Cucumis sativus
3.4.11.2 1.72
-
 Gly-4-nitroanilide pH 8.0, 37°C Cucumis sativus
3.4.11.2 7.83
-
 L-Ser-2-naphthylamide pH 8.0, 37°C Cucumis sativus
3.4.11.2 9.23
-
 L-Leu-4-nitroanilide pH 8.0, 37°C Cucumis sativus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.2 additional information metallopeptidase Cucumis sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.2 95000
-
 2 * 95000, SDS-PAGE Cucumis sativus
3.4.11.2 200000
-
 gel filtration Cucumis sativus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Cucumis sativus
-
 var. suyo
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.2 native enzyme 365fold from leaves by ammonium sulfate fractionation, ion exchange chromatography, hydrophobic interaction chromatography, hydroxyapatite chromatography, again ion exchange chromatography, and gel filtration Cucumis sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.2 leaf
-
 Cucumis sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.2 6.86
-
 purified enzyme, substrate L-Ala-2-naphthylamide Cucumis sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 Gly-4-nitroanilide + H2O
-
 Cucumis sativus Gly + 4-nitroaniline
-
 ?
3.4.11.2 L-Ala-2-naphthylamide + H2O best substrate Cucumis sativus L-Ala + 2-naphthylamine
-
 ?
3.4.11.2 L-Ala-4-nitroanilide + H2O
-
 Cucumis sativus L-Ala + 4-nitroaniline
-
 ?
3.4.11.2 L-Arg-2-naphthylamide + H2O
-
 Cucumis sativus L-Arg + 2-naphthylamine
-
 ?
3.4.11.2 L-Leu-4-nitroanilide + H2O very low activity Cucumis sativus L-Leu + 4-nitroaniline
-
 ?
3.4.11.2 L-Lys-2-naphthylamide + H2O
-
 Cucumis sativus L-Lys + 2-naphthylamine
-
 ?
3.4.11.2 L-Met-4-nitroanilide + H2O
-
 Cucumis sativus L-Met + 4-nitroaniline
-
 ?
3.4.11.2 L-Ser-2-naphthylamide + H2O low activity Cucumis sativus L-Ser + 2-naphthylamine
-
 ?
3.4.11.2 additional information the enzyme prefers substrates with N-terminal L-Ala residues Cucumis sativus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.2 dimer 2 * 95000, SDS-PAGE Cucumis sativus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.2 APase2
-
 Cucumis sativus
3.4.11.2 More the enzyme probably belongs to the M1 family of peptidases Cucumis sativus
3.4.11.2 plant aminopeptidase N
-
 Cucumis sativus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.2 37
-
 assay at Cucumis sativus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.2 0.052 2.1 L-Met-4-nitroanilide pH 8.0, 37°C Cucumis sativus
3.4.11.2 6.7
-
 L-Leu-4-nitroanilide pH 8.0, 37°C Cucumis sativus
3.4.11.2 9.72
-
 L-Met-4-nitroanilide pH 8.0, 37°C Cucumis sativus
3.4.11.2 19.3
-
 L-Arg-2-naphthylamide pH 8.0, 37°C Cucumis sativus
3.4.11.2 23.1
-
 L-Lys-2-naphthylamide pH 8.0, 37°C Cucumis sativus
3.4.11.2 34.3
-
 L-Ala-4-nitroanilide pH 8.0, 37°C Cucumis sativus
3.4.11.2 45.2
-
 Gly-4-nitroanilide pH 8.0, 37°C Cucumis sativus
3.4.11.2 87
-
 L-Ser-2-naphthylamide pH 8.0, 37°C Cucumis sativus
3.4.11.2 149
-
 L-Ala-2-naphthylamide pH 8.0, 37°C Cucumis sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.2 8 9
-
 Cucumis sativus