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Literature summary extracted from

  • Adachi, O.; Ano, Y.; Moonmangmee, D.; Shinagawa, E.; Toyama, H.; Theeragool, G.; Lotong, N.; Matsushita, K.
    Crystallization and properties of NADPH-dependent L-sorbose reductase from Gluconobacter melanogenus IFO 3294 (1999), Biosci. Biotechnol. Biochem., 63, 2137-2143.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.289
-
 Gluconobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.289 0.032
-
 NADPH pH 6.0, 25°C Gluconobacter oxydans
1.1.1.289 35
-
 L-sorbose pH 6.0, 25°C Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.289 cytosol
-
 Gluconobacter oxydans 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.289 30000
-
 2 * 30000, SDS-PAGE Gluconobacter oxydans
1.1.1.289 60000
-
 gel filtration Gluconobacter oxydans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.289 Gluconobacter oxydans
-
-
-
1.1.1.289 Gluconobacter oxydans IFO 3294
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.289
-
 Gluconobacter oxydans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.289 77
-
 pH 6.0, 25°C, substrate L-sorbose Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.289 D-sorbitol + NADP+
-
 Gluconobacter oxydans L-sorbose + NADPH + H+
-
 r
1.1.1.289 D-sorbitol + NADP+
-
 Gluconobacter oxydans IFO 3294 L-sorbose + NADPH + H+
-
 r
1.1.1.289 L-sorbose + NADPH + H+
-
 Gluconobacter oxydans D-sorbitol + NADP+
-
 r
1.1.1.289 L-sorbose + NADPH + H+
-
 Gluconobacter oxydans IFO 3294 D-sorbitol + NADP+
-
 r
1.1.1.289 additional information highly specific for D-sorbitol and L-sorbose. Reaction rate in L-sorbose reduction highly predominates over L-sorbitol oxidation over a wide pH range Gluconobacter oxydans ?
-
 ?
1.1.1.289 additional information highly specific for D-sorbitol and L-sorbose. Reaction rate in L-sorbose reduction highly predominates over L-sorbitol oxidation over a wide pH range Gluconobacter oxydans IFO 3294 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.289 dimer 2 * 30000, SDS-PAGE Gluconobacter oxydans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.289 L-sorbose reductase
-
 Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.289 6
-
 reduction of L-sorbose Gluconobacter oxydans
1.1.1.289 8
-
 oxidation of D-sorbitol Gluconobacter oxydans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.289 5 7 reduction of L-sorbose Gluconobacter oxydans
1.1.1.289 7 9 oxidation of D-sorbitol Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.289 additional information no cofactor: NAD+, NADH Gluconobacter oxydans
1.1.1.289 NADP+
-
 Gluconobacter oxydans
1.1.1.289 NADPH
-
 Gluconobacter oxydans