Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kaur, G.; Kumar, S.; Satyanarayana, T.
    Production, characterization and application of a thermostable polygalacturonase of a thermophilic mould Sporotrichum thermophils Apinis (2004), Biores. Technol., 94, 239-243.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.15 nutrition potential application of the enzyme in fruit juice extraction Thermothelomyces heterothallicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.15 2-mercaptoethanol
-
 Thermothelomyces heterothallicus
3.2.1.15 Ca2+ 5 mM Thermothelomyces heterothallicus
3.2.1.15 Cu2+ 5 mM Thermothelomyces heterothallicus
3.2.1.15 Mg2+ strong inhibition Thermothelomyces heterothallicus
3.2.1.15 SDS
-
 Thermothelomyces heterothallicus
3.2.1.15 Triton X-100 slight inhibition Thermothelomyces heterothallicus
3.2.1.15 Tween 80 slight inhibition Thermothelomyces heterothallicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.15 additional information
-
 additional information the KM-value for pectin is 0.416 mg/ml Thermothelomyces heterothallicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.15 Ca2+ 1 mM, stimulates Thermothelomyces heterothallicus
3.2.1.15 Cu2+ 1 mM, stimulates Thermothelomyces heterothallicus
3.2.1.15 Fe2+ 1 mM and 5 mM, stimulates Thermothelomyces heterothallicus
3.2.1.15 Mn2+ 1 mM and 5 mM, stimulates Thermothelomyces heterothallicus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Thermothelomyces heterothallicus
-
 Apinis
-
3.2.1.15 Thermothelomyces heterothallicus Apinis
-
 Apinis
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15
-
 Thermothelomyces heterothallicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 pectin + H2O
-
 Thermothelomyces heterothallicus ?
-
 ?
3.2.1.15 pectin + H2O
-
 Thermothelomyces heterothallicus Apinis ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.15 55
-
-
 Thermothelomyces heterothallicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.15 50 70 50°C: about 55% of maximal activity, 70°C: about 50% of maximal activity Thermothelomyces heterothallicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.15 65
-
 t1/2: 4 h Thermothelomyces heterothallicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 7
-
-
 Thermothelomyces heterothallicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.15 4 8 pH 4.0: about 50% of maximal activity, pH 8.0: about 50% of maximal activity Thermothelomyces heterothallicus