EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.21.B10 | the precursor form of human kallikrein 6 is overexpressed in Pichia pastoris and is autoprocessed to an active but unstable mature enzyme that subsequently yields the inactive, self-cleavage product, hK6(D81-K244) | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.21.B10 | R80Q | mutation stabilizes the activity of the mature enzyme | Homo sapiens |
3.4.21.B10 | S197A | mutation results in complete loss of hK6 proteolytic activity | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.B10 | Bovine pancreatic trypsin inhibitor | weak | Homo sapiens | |
3.4.21.B10 | Bowman-Birk inhibitor | weak | Homo sapiens | |
3.4.21.B10 | Soybean trypsin inhibitor | weak | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.B10 | plasminogen + H2O | Homo sapiens | the enzyme is regulated by an autoactivation/autoinactivation mechanism. Mature hK67 displays a trypsin-like activity against human plasminogen, the putative physiological substrate | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.B10 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.B10 | recombinant | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.B10 | Gelatin + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.B10 | additional information | hK6 does not hydrolyze the Nalpha-tosyl-L-Arg methyl ester | Homo sapiens | ? | - |
? | |
3.4.21.B10 | Nalpha-benzoyl-L-Arg + H2O | - |
Homo sapiens | benzoic acid + L-Arg | - |
? | |
3.4.21.B10 | plasminogen + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.B10 | plasminogen + H2O | the enzyme is regulated by an autoactivation/autoinactivation mechanism. Mature hK67 displays a trypsin-like activity against human plasminogen, the putative physiological substrate | Homo sapiens | ? | - |
? |