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Literature summary extracted from

  • Bayes, A.; Tsetsenis, T.; Ventura, S.; Vendrell, J.; Aviles, F.X.; Sotiropoulou, G.
    Human kallikrein 6 activity is regulated via an autoproteolytic mechanism of activation/inactivation (2004), Biol. Chem., 385, 517-524.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B10 the precursor form of human kallikrein 6 is overexpressed in Pichia pastoris and is autoprocessed to an active but unstable mature enzyme that subsequently yields the inactive, self-cleavage product, hK6(D81-K244) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.B10 R80Q mutation stabilizes the activity of the mature enzyme Homo sapiens
3.4.21.B10 S197A mutation results in complete loss of hK6 proteolytic activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B10 Bovine pancreatic trypsin inhibitor weak Homo sapiens
3.4.21.B10 Bowman-Birk inhibitor weak Homo sapiens
3.4.21.B10 Soybean trypsin inhibitor weak Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.B10 plasminogen + H2O Homo sapiens the enzyme is regulated by an autoactivation/autoinactivation mechanism. Mature hK67 displays a trypsin-like activity against human plasminogen, the putative physiological substrate ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B10 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B10 recombinant Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B10 Gelatin + H2O
-
Homo sapiens ?
-
?
3.4.21.B10 additional information hK6 does not hydrolyze the Nalpha-tosyl-L-Arg methyl ester Homo sapiens ?
-
?
3.4.21.B10 Nalpha-benzoyl-L-Arg + H2O
-
Homo sapiens benzoic acid + L-Arg
-
?
3.4.21.B10 plasminogen + H2O
-
Homo sapiens ?
-
?
3.4.21.B10 plasminogen + H2O the enzyme is regulated by an autoactivation/autoinactivation mechanism. Mature hK67 displays a trypsin-like activity against human plasminogen, the putative physiological substrate Homo sapiens ?
-
?