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Literature summary extracted from

  • Girish, K.S.; Shashidharamurthy, R.; Nagaraju, S.; Gowda, T.V.; Kemparaju, K.
    Isolation and characterization of hyaluronidase a "spreading factor" from Indian cobra (Naja naja) venom (2004), Biochimie, 86, 193-202.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.35 extracellular venom Naja naja
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.35 70410
-
 mass spectrometry, isozyme NNH1 Naja naja

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.35 hyaluronan + H2O Naja naja absolutely specific for tetrasaccharides units of hyaluronan
-
 ?
3.2.1.35 additional information Naja naja the enzyme indirectly potentiates the myotoxicity of VRV-PL-VIII, a phospholipolytic myotoxin, and also the hemorrhagic potency of a hemorrhagic complex-I ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.35 Naja naja
-
 two isozymes NNH1 and NNH2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.35 isozymes NNH1 and NNH2 33fold and 26fold, respectively, from venom by gel filtration and ion exchange chromatography Naja naja

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.35 venom
-
 Naja naja
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.35 additional information
-
 purified isozymes NNH1 and NNH2 Naja naja

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.35 hyaluronan + H2O absolutely specific for Naja naja tetrasaccharides units of hyaluronan
-
 ?
3.2.1.35 hyaluronan + H2O absolutely specific for, cleavage of beta-1,4-glycosidic linkages, of human skin, extracellular matrix Naja naja tetrasaccharides units of hyaluronan
-
 ?
3.2.1.35 additional information the enzyme indirectly potentiates the myotoxicity of VRV-PL-VIII, a phospholipolytic myotoxin, and also the hemorrhagic potency of a hemorrhagic complex-I Naja naja ?
-
 ?
3.2.1.35 additional information no activity with chondroitin sulfates, heparin, chitosan, 4-nitrophenyl-N-acetyl-beta-D-glucosaminide, or 4-nitrophenyl-beta-D-glucuronide Naja naja ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.35 hyaluronidase
-
 Naja naja
3.2.1.35 NNH1
-
 Naja naja
3.2.1.35 NNH2
-
 Naja naja
3.2.1.35 spreading factor
-
 Naja naja

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.35 37
-
 assay at Naja naja

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.35 5
-
 assay at Naja naja

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.35 Naja naja isoelectric focusing
-
 9.2