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Literature summary extracted from

  • Funami, J.; Yoshikane, Y.; Kobayashi, H.; Yokochi, N.; Yuan, B.; Iwasaki, K.; Ohnishi, K.; Yagi, T.
    4-Pyridoxolactonase from a symbiotic nitrogen-fixing bacterium Mesorhizobium loti: cloning, expression, and characterization (2005), Biochim. Biophys. Acta, 1753, 234-239.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.27
-
 Mesorhizobium loti

General Stability

EC Number General Stability Organism
3.1.1.27 enzyme shows very low activity in Tris-HCl bufffers Mesorhizobium loti

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.27 5-Pyridoxolactone competitive Mesorhizobium loti
3.1.1.27 Hg2+ 0.1 mM, complete inhibition Mesorhizobium loti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.27 0.008
-
 4-Pyridoxolactone pH 7.5 Mesorhizobium loti
3.1.1.27 0.319
-
 4-pyridoxate pH 7.5 Mesorhizobium loti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.27 Zn2+ one mole per mole of subunit, tightly bound to the protein Mesorhizobium loti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.27 29855
-
 2 * 31000, SDS-PAGE, 2 * 29855, calculated Mesorhizobium loti
3.1.1.27 31000
-
 2 * 31000, SDS-PAGE, 2 * 29855, calculated Mesorhizobium loti
3.1.1.27 59000
-
 gel filtration Mesorhizobium loti

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.27 Mesorhizobium loti
-
 overexpression in Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.27 4-pyridoxate
-
 Mesorhizobium loti 4-pyridoxolactone + H2O
-
 r
3.1.1.27 4-pyridoxolactone + H2O
-
 Mesorhizobium loti 4-pyridoxate
-
 r
3.1.1.27 N-hexanoyl-D,L-homoserine lactone + H2O 41% of the activity with 4-pyridoxolactone Mesorhizobium loti N-hexanoyl-D,L-homoserine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.27 dimer 2 * 31000, SDS-PAGE, 2 * 29855, calculated Mesorhizobium loti
3.1.1.27 More N-terminal amino acid sequence Mesorhizobium loti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.27 60 70
-
 Mesorhizobium loti

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.27 50
-
 10 min, stable Mesorhizobium loti
3.1.1.27 55
-
 10 min, 64% residual activity Mesorhizobium loti
3.1.1.27 60
-
 10 min, 14% residual activity Mesorhizobium loti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.27 1.4
-
 4-pyridoxate pH 7.5 Mesorhizobium loti
3.1.1.27 12.2
-
 4-Pyridoxolactone pH 7.5 Mesorhizobium loti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.27 7.5
-
-
 Mesorhizobium loti

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.27 5
-
 36% of maximum activity Mesorhizobium loti
3.1.1.27 9.5
-
 10% of maximum activity Mesorhizobium loti

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.27 0.15
-
 5-Pyridoxolactone pH 7.5 Mesorhizobium loti