Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Krastanova, I.; Guarnaccia, C.; Zahariev, S.; Degrassi, G.; Lamba, D.
    Heterologous expression, purification, crystallization, X-ray analysis and phasing of the acetyl xylan esterase from Bacillus pumilus (2005), Biochim. Biophys. Acta, 1748, 222-230.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.72 heterologous expression Bacillus pumilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.72 recombinant wild-type enzyme, hanging drop vapor diffusion method, in a micro-batch experiment. Rod-like diffraction quality crystals are obtained using 10% PEG 6000, 0.1 M MES pH 6.0 and a wide range of LiCl concentrations (0.2-1.0 M) as precipitating agent. Two different crystal forms, bith belonging to space group P2(1), are characterized, diffracting X-rays to 2.5 and 1.9 A resolution. 12 molecules in the asymmetric unit of either crystal forms are arranged as two doughnut-like hexamers, each one encompassing a local 32 symmewtry. Catalytic inactive mutant S181A is also crystallized Bacillus pumilus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.72 S181A inactive mutant Bacillus pumilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.72 38000
-
6 * or 7 * 38000, SDS-PAGE Bacillus pumilus
3.1.1.72 260000
-
gel filtration Bacillus pumilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.72 additional information Bacillus pumilus accessory enzyme in plant cell wall hemicellulose biodegradation pathway ?
-
?
3.1.1.72 additional information Bacillus pumilus PS213 accessory enzyme in plant cell wall hemicellulose biodegradation pathway ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.72 Bacillus pumilus
-
PS213
-
3.1.1.72 Bacillus pumilus PS213
-
PS213
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.72
-
Bacillus pumilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.72 186.5
-
-
Bacillus pumilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.72 additional information accessory enzyme in plant cell wall hemicellulose biodegradation pathway Bacillus pumilus ?
-
?
3.1.1.72 additional information accessory enzyme in plant cell wall hemicellulose biodegradation pathway Bacillus pumilus PS213 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.72 oligomer 6 * or 7 * 38000, SDS-PAGE Bacillus pumilus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.72 AXE
-
Bacillus pumilus