BRENDA - Enzyme Database

The first archaeal agmatinase from anaerobic hyperthermophilic archaeon Pyrococcus horikoshii: cloning, expression, and characterization

Goda, S.; Sakuraba, H.; Kawarabayasi, Y.; Ohshima, T.; Biochim. Biophys. Acta 1748, 110-115 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.3.11
expression in Escherichia coli. Mainly obtained as inactive inclusion body in Escherichia coli
Pyrococcus horikoshii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.11
putrescine
-
Pyrococcus horikoshii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.11
0.53
-
agmatine
50C, pH 11.3
Pyrococcus horikoshii
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.3.11
Ca2+
enzyme loses activity by dialysis against a buffer without cations, but the activity is recovered by the addition of divalent cations. CoCl2 is the most effective, and CaCl2, MnCl2, ZnCl2 or MgCl2 is replaced to some extent instead of CoCl2
Pyrococcus horikoshii
3.5.3.11
Co2+
enzyme loses activity by dialysis against a buffer without cations, but the activity is recovered by the addition of divalent cations. CoCl2 is the most effective, and CaCl2, MnCl2, ZnCl2 or MgCl2 is replaced to some extent instead of CoCl2
Pyrococcus horikoshii
3.5.3.11
Mg2+
enzyme loses activity by dialysis against a buffer without cations, but the activity is recovered by the addition of divalent cations. CoCl2 is the most effective, and CaCl2, MnCl2, ZnCl2 or MgCl2 is replaced to some extent instead of CoCl2
Pyrococcus horikoshii
3.5.3.11
Mn2+
enzyme loses activity by dialysis against a buffer without cations, but the activity is recovered by the addition of divalent cations. CoCl2 is the most effective, and CaCl2, MnCl2, ZnCl2 or MgCl2 is replaced to some extent instead of CoCl2
Pyrococcus horikoshii
3.5.3.11
Zn2+
enzyme loses activity by dialysis against a buffer without cations, but the activity is recovered by the addition of divalent cations. CoCl2 is the most effective, and CaCl2, MnCl2, ZnCl2 or MgCl2 is replaced to some extent instead of CoCl2
Pyrococcus horikoshii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.11
31000
-
4 * 31000, SDS-PAGE
Pyrococcus horikoshii
3.5.3.11
145000
-
gel filtration
Pyrococcus horikoshii
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
3.5.3.11
dimethyl sulfoxide
10%, no loss of activity at 50C
Pyrococcus horikoshii
3.5.3.11
Ethanol
10%, no loss of activity at 50C
Pyrococcus horikoshii
3.5.3.11
Methanol
10%, no loss of activity at 50C
Pyrococcus horikoshii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.11
Pyrococcus horikoshii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.3.11
recombinant
Pyrococcus horikoshii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.11
agmatine + H2O
-
664200
Pyrococcus horikoshii
putrescine + urea
-
-
-
?
3.5.3.11
additional information
no activity with arginine
664200
Pyrococcus horikoshii
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.5.3.11
tetramer
4 * 31000, SDS-PAGE
Pyrococcus horikoshii
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.3.11
100
-
-
Pyrococcus horikoshii
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.5.3.11
80
-
10 min, stable
Pyrococcus horikoshii
3.5.3.11
90
-
10 min, 53% loss of activity
Pyrococcus horikoshii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.11
11
-
-
Pyrococcus horikoshii
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.3.11
9.5
12.5
pH 9.5: about 50% of maximal activity, pH 12.5: about 85% of maximal activity
Pyrococcus horikoshii
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.3.11
1.8
-
putrescine
50C, pH 11.3
Pyrococcus horikoshii
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.11
expression in Escherichia coli. Mainly obtained as inactive inclusion body in Escherichia coli
Pyrococcus horikoshii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.11
putrescine
-
Pyrococcus horikoshii
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.3.11
1.8
-
putrescine
50C, pH 11.3
Pyrococcus horikoshii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.11
0.53
-
agmatine
50C, pH 11.3
Pyrococcus horikoshii
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.3.11
Ca2+
enzyme loses activity by dialysis against a buffer without cations, but the activity is recovered by the addition of divalent cations. CoCl2 is the most effective, and CaCl2, MnCl2, ZnCl2 or MgCl2 is replaced to some extent instead of CoCl2
Pyrococcus horikoshii
3.5.3.11
Co2+
enzyme loses activity by dialysis against a buffer without cations, but the activity is recovered by the addition of divalent cations. CoCl2 is the most effective, and CaCl2, MnCl2, ZnCl2 or MgCl2 is replaced to some extent instead of CoCl2
Pyrococcus horikoshii
3.5.3.11
Mg2+
enzyme loses activity by dialysis against a buffer without cations, but the activity is recovered by the addition of divalent cations. CoCl2 is the most effective, and CaCl2, MnCl2, ZnCl2 or MgCl2 is replaced to some extent instead of CoCl2
Pyrococcus horikoshii
3.5.3.11
Mn2+
enzyme loses activity by dialysis against a buffer without cations, but the activity is recovered by the addition of divalent cations. CoCl2 is the most effective, and CaCl2, MnCl2, ZnCl2 or MgCl2 is replaced to some extent instead of CoCl2
Pyrococcus horikoshii
3.5.3.11
Zn2+
enzyme loses activity by dialysis against a buffer without cations, but the activity is recovered by the addition of divalent cations. CoCl2 is the most effective, and CaCl2, MnCl2, ZnCl2 or MgCl2 is replaced to some extent instead of CoCl2
Pyrococcus horikoshii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.11
31000
-
4 * 31000, SDS-PAGE
Pyrococcus horikoshii
3.5.3.11
145000
-
gel filtration
Pyrococcus horikoshii
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
3.5.3.11
dimethyl sulfoxide
10%, no loss of activity at 50C
Pyrococcus horikoshii
3.5.3.11
Ethanol
10%, no loss of activity at 50C
Pyrococcus horikoshii
3.5.3.11
Methanol
10%, no loss of activity at 50C
Pyrococcus horikoshii
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.11
recombinant
Pyrococcus horikoshii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.11
agmatine + H2O
-
664200
Pyrococcus horikoshii
putrescine + urea
-
-
-
?
3.5.3.11
additional information
no activity with arginine
664200
Pyrococcus horikoshii
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.3.11
tetramer
4 * 31000, SDS-PAGE
Pyrococcus horikoshii
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.3.11
100
-
-
Pyrococcus horikoshii
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.5.3.11
80
-
10 min, stable
Pyrococcus horikoshii
3.5.3.11
90
-
10 min, 53% loss of activity
Pyrococcus horikoshii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.11
11
-
-
Pyrococcus horikoshii
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.3.11
9.5
12.5
pH 9.5: about 50% of maximal activity, pH 12.5: about 85% of maximal activity
Pyrococcus horikoshii