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Literature summary extracted from

  • Puchart, V.; Vrsanska, M.; Svoboda, P.; Pohl, J.; Ogel, Z.B.; Biely, P.
    Purification and characterization of two forms of endo-beta-1,4-mannanase from a thermotolerant fungus, Aspergillus fumigatus IMI 385708 (formerly Thermomyces lanuginosus IMI 158749) (2004), Biochim. Biophys. Acta, 1674, 239-250.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.78 nutrition pulp production Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.78 3.07
-
 low viscosity locust bean galactomannan isozyme MAN I Aspergillus fumigatus
3.2.1.78 3.12
-
 low viscosity locust bean galactomannan isozyme MAN II Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 60000
-
 isozyme MAN I, SDS-PAGE Aspergillus fumigatus
3.2.1.78 63000
-
 isozyme MAN II, SDS-PAGE Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Aspergillus fumigatus
-
 strain IMI 385708, formerly Thermomyces lanuginosus strain IMI 158749
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 fast flow column chromatography Aspergillus fumigatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.78 culture fluid
-
 Aspergillus fumigatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 additional information
-
 1638 A550/min/mg optical path 1cm,isozyme MAN I, OBR-LBG Aspergillus fumigatus
3.2.1.78 additional information
-
 1763 A550/min/mg optical path 1cm, isozyme MAN II, OBR-LBG Aspergillus fumigatus
3.2.1.78 additional information
-
 no activity against 4-nitrophenyl-alpha and -beta-mannopyranosides Aspergillus fumigatus
3.2.1.78 4.6
-
 crude medium Aspergillus fumigatus
3.2.1.78 45.7
-
 isozyme MAN I, after 9.9fold purification with DEAE Sepharose chromatography Aspergillus fumigatus
3.2.1.78 56.7
-
 isozyme MAN II, after 12.3fold purification with DEAE Sepharose chromatography Aspergillus fumigatus
3.2.1.78 190
-
 isozyme MAN I, mannan A Aspergillus fumigatus
3.2.1.78 215
-
 isozyme MAN II, mannan A Aspergillus fumigatus
3.2.1.78 225.4
-
 isozyme MAN II, after 49fold purification with Phenyl Sepharose chromatography Aspergillus fumigatus
3.2.1.78 294
-
 isozyme MAN I, guar gum Aspergillus fumigatus
3.2.1.78 317
-
 isozyme MAN II, guar gum Aspergillus fumigatus
3.2.1.78 471
-
 isozyme MAN I, after 102.4fold purification with Phenyl Sepharose chromatography Aspergillus fumigatus
3.2.1.78 562
-
 isozyme MAN I, locust bean gum Aspergillus fumigatus
3.2.1.78 627
-
 isozyme MAN II, locust bean gum Aspergillus fumigatus
3.2.1.78 663
-
 isozyme MAN I, low viscosity locust bean galactomannan Aspergillus fumigatus
3.2.1.78 751
-
 isozyme MAN II, low viscosity locust bean galactomannan Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 guar gum + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.2.1.78 locust bean gum + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.2.1.78 low viscosity locust bean galactomannan + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.2.1.78 mannan A + H2O
-
 Aspergillus fumigatus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 endo-beta-1,4-mannanase
-
 Aspergillus fumigatus
3.2.1.78 MAN I isozyme, major form Aspergillus fumigatus
3.2.1.78 Man II isozyme, minor form Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 60
-
 at pH 4.5 Aspergillus fumigatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.78 60 65
-
 Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 55
-
 stable up to 55°C Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.78 1935
-
 low viscosity locust bean galactomannan isozyme MAN I Aspergillus fumigatus
3.2.1.78 2246
-
 low viscosity locust bean galactomannan isozyme MAN II Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 4.5
-
 at 60°C Aspergillus fumigatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.78 4 5
-
 Aspergillus fumigatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 4.5 8.5
-
 Aspergillus fumigatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.78 Aspergillus fumigatus isozyme MAN II 4.9 4.75
3.2.1.78 Aspergillus fumigatus isozyme MAN I 5.2 4.9